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Aminoethoxyvinylglycine

Manufactured by Merck Group
Sourced in China

Aminoethoxyvinylglycine is a laboratory product utilized as an analytical standard for various chemical and biological applications. It serves as a reference compound in research and development settings, supporting accurate identification and quantification of related substances.

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2 protocols using aminoethoxyvinylglycine

1

Nitrate and Rhizobium Regulation of M. truncatula Gene Expression

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To analyze nitrate-induced gene expression, M. truncatula wild-type (R108), cra2, nin-2, or nlp1 seedlings were grown on BNM or FP plates supplemented with 50 nM aminoethoxyvinylglycine (Sigma-Aldrich) for 3 days, then transferred to plates with BNM or FP containing 10 mM KCl or KNO3 for another 3 days. To analyze rhizobium-induced gene expression, R108, nin-2, or nlp1 seedlings were grown on BNM or FP agar plates for 3 days, then inoculated with Sm1021 (OD600 ≈ 0.05) and grown for another 3 days. Total RNA was extracted from M. truncatula roots using the TRIpure isolation reagent (Aidlab, Beijing, China) according to the manufacturer's instructions. RNA was quantified using a NanoDrop 2000 instrument (Thermo Fisher, Waltham, MA, USA) and then reverse transcribed into cDNA using the TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen, Beijing, China). Gene transcript levels were analyzed by real-time PCR using TB Green Premix Ex Taq (Takara, Dalian, China) with the StepOnePlus PCR system (Thermo Fisher, Waltham, MA, USA). Relative transcript levels were normalized against that of the reference gene (MtUBQ10). Statistical significance based on three biological replicates was calculated using the 2−ΔΔCt method. The primers used for qRT-PCR are listed in Supplemental Table 1.
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2

Effect of Phytohormones and Inhibitors on Seed Germination

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Kinetin (KT), lovastatin, and aminoethoxyvinylglycine (AVG) were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China). Each chemical was dissolved in dimethyl sulfoxide (DMSO) and diluted to the required concentration with distilled water. The pH of all treatment solutions was adjusted to 6.5, and all solutions had the same concentration of DMSO (0.01% v/v). KT was used as a synthetic CK, lovastatin was used as a CK biosynthesis inhibitor [46 (link),47 (link),48 (link)], and AVG was used as an ethylene biosynthesis inhibitor [15 (link),49 (link),50 (link)]. Germinated seeds were incubated in one of these solutions or in distilled water as a control. All treatment solutions were refreshed every 2 days.
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