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Leptomycin b l2913

Manufactured by Merck Group
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Leptomycin B (L2913) is a laboratory compound supplied by Merck Group. It is a natural product isolated from the bacterium Streptomyces. Leptomycin B is known to inhibit the nuclear export receptor protein Exportin 1 (XPO1 or CRM1).

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Lab products found in correlation

Puromycin dihydrochloride, by Merck Group (2 mentions) Polybrene tr 1003 g, by Merck Group (2 mentions) Z vad ome fmk ab120487, by Abcam (2 mentions) N acetyl l cysteine a9165, by Merck Group (2 mentions) Chloroquine diphosphate salt c6628, by Merck Group (2 mentions) Anti lyn, by Santa Cruz Biotechnology (1 mentions) Calyculin a, by Cell Signaling Technology (1 mentions) Anti β actin a1978, by Merck Group (1 mentions) Anti ha tag sc805, by Santa Cruz Biotechnology (1 mentions) Cycloheximide c1988, by Merck Group (1 mentions) Mg132 a2585, by Apexbio (1 mentions) Rhodamine phalloidin phdr1, by Cytoskeleton (1 mentions) Anti flag m2 f1804, by Merck Group (1 mentions)

5 protocols using leptomycin b l2913

1

Selective Inhibitors for Cell Research

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MG132 (ab141003), bortezomib (ab142123) and Z-VAD(OMe)-FMK (ab120487) were obtained from Abcam (Cambridge, UK). Chloroquine diphosphate salt (C6628), Cyclohexamide (C7698), Leptomycin B (L2913), N-Acetyl-L-cysteine (A9165), Doxycycline hyclate (D9891), G418 sulfate (345810), 12-O-Tetradecanoylphorbol-13-acetate or TPA (P1585), sodium butyrate (B5887), Polybrene (TR-1003-G) and Puromycin dihydrochloride (P8833) were brought from Sigma-Aldrich Corp. (St. Louis, MO, USA).
Gain C., Malik S., Bhattacharjee S., Ghosh A., Robertson E.S., Das B.B, & Saha A. (2020). Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway. PLoS Pathogens, 16(2), e1008105.
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2

Selective Inhibitors for Cell Research

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MG132 (ab141003), bortezomib (ab142123) and Z-VAD(OMe)-FMK (ab120487) were obtained from Abcam (Cambridge, UK). Chloroquine diphosphate salt (C6628), Cyclohexamide (C7698), Leptomycin B (L2913), N-Acetyl-L-cysteine (A9165), Doxycycline hyclate (D9891), G418 sulfate (345810), 12-O-Tetradecanoylphorbol-13-acetate or TPA (P1585), sodium butyrate (B5887), Polybrene (TR-1003-G) and Puromycin dihydrochloride (P8833) were brought from Sigma-Aldrich Corp. (St. Louis, MO, USA).
Gain C., Malik S., Bhattacharjee S., Ghosh A., Robertson E.S., Das B.B, & Saha A. (2020). Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway. PLoS Pathogens, 16(2), e1008105.
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3

Antibodies for Ankrd54 Characterization

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HEK293T cells were maintained in Delbocco’s modified Eagle’s medium (DMEM) with 10% fetal calf serum (FCS). Polyclonal rabbit antibodies were raised against murine Ankrd54 using the purified full-length protein fused to GST, as the antigen, and high titer serum was purified on protein-A beads as described previously[9 (link)]. Additional antibodies used for immunoblotting were anti-Lyn (Santa-Cruz Biotechnology, sc-15 and sc-7274); anti-β-actin (AC-15, ab6276, Abcam, Cambridge, United Kingdom); anti-Histone H3 (H0164, Sigma-Aldrich); and anti-14-3-3ζ (ab51129, Abcam). TRITC-labeled phalloidin (P1591, Sigma-Aldrich) was used to visualize filamentous actin (F-actin). Phorbol 12-myristate 13-acetate (PMA, P8139), staurosporine (S4400) and leptomycin B (L2913) were purchased from Sigma-Aldrich, calyculin A (#9902) was purchased from Cell Signaling Technologies, and Phos-tag™-Acrylamide TM (ALL-107) was purchased from Wako Laboratory Chemicals (Japan).
Samuels A.L., Louw A., Zareie R, & Ingley E. (2017). Control of nuclear-cytoplasmic shuttling of Ankrd54 by PKCδ. World Journal of Biological Chemistry, 8(3), 163-174.
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4

Maintenance of HeLa and HCT116 Cells

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HeLa cervical adenocarcinoma cells (CCL-2, ATCC) and HCT116 cells (wild type and DICER−/−) were maintained in DMEM supplemented with 10% FCS. HCT116 and DICER−/− cells were a kind gift of Dr. V. Velculescu (Johns Hopkins University, Baltimore, MD). All cell culture reagents were obtained from Invitrogen. Actinomycin D (ActD, A1410) and Leptomycin B (L2913) were from Sigma-Aldrich.
Bai B., Yegnasubramanian S., Wheelan S.J, & Laiho M. (2014). RNA-Seq of the Nucleolus Reveals Abundant SNORD44-Derived Small RNAs. PLoS ONE, 9(9), e107519.
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5

Antibody Characterization and Reagent Validation

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Anti-HDAC1 antibody was previously generated in our laboratory69 (link). Anti-FLAG M2 (F1804) and anti-β-actin (A1978) antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, HRP-60004) antibody was from Proteintech (Rosemont, IL). Anti-Lamin A (sc-20680) and anti-HA tag (sc-805) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-EGFR (A300-388A) antibody was from Bethyl Laboratories (Montgomery, TX). Anti-phospho-EGFR (Y1173) (AF1095) antibody was from R&D Systems (Minneapolis, MN). Anti-Bim (2876) and anti-Myc tag (9B11, 2276) antibodies were from Cell Signaling Technology (Danvers, MA). Phosphotyrosine antibody cocktail (13-6620) was from Invitrogen (Thermo Fisher Scientific, Waltham, MA). Mouse (NA931) and Rabbit (NA934) IgG HRP-linked antibodies were purchased from GE Healthcare (Chicago, IL).
Rhodamine Phalloidin (PHDR1) was from Cytoskeleton (Denver, CO). Epidermal Growth Factor (EGF; cat. 236-EG) was purchased from R&D Systems. Gefitinib (ZD1839) was from Cayman Chemical Company (Ann Arbor, MI). AG-1478 (A8357) and MG-132 (A2585) were from ApexBio (Houston, TX). Cycloheximide (C1988) and leptomycin B (L2913) were purchased from Sigma-Aldrich.
Bahl S., Ling H., Acharige N.P., Santos-Barriopedro I., Pflum M.K, & Seto E. (2021). EGFR phosphorylates HDAC1 to regulate its expression and anti-apoptotic function. Cell Death & Disease, 12(5), 469.
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