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2 protocols using 3 4 morpholino propane sulfonic acid mops

1

PEG-Polymer Characterization Protocols

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Poly(ethylene glycol) with a molecular weight of 2000 g/mol (PEG2000) was obtained from Fluka. Poly(ethylene glycol-block-caprolactone) (PEG-CL; AK073; 70411STR-B) and poly(ethylene glycol-block-polylactide) (PEG-LA; AK009; 190813RAI-B) were obtained from Polyscitech and used for BSA ITC and CD experiments. Poly(ethylene glycol-block-caprolactone) (PEG-CL; AK073; 40319SMS-A) and poly(ethylene glycol-block-polylactide) (PEG-LA; AK009; 180329YSK-A) were obtained from Polyscitech and used for protein fluorescence and HSA ITC and CD experiments. 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (PEG-DSPE) was obtained from Avanti Polar Lipids. Fatty acid-free bovine serum albumin (BSA) was obtained from Millipore Sigma. Fatty acid-free human serum albumin and pyrene were obtained from Sigma-Aldrich. Acetone, sodium chloride, sodium phosphate dibasic anhydrous, sodium phosphate monobasic, 3-(4-morpholino)propane sulfonic acid (MOPS), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and sodium dodecyl sulfate (SDS) were obtained from Fisher Scientific. Regenerated cellulose dialysis membranes with a molecular weight cutoff of 3500 g/mol were obtained from Spectrum Laboratories.
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2

Protein-Polymer Interaction Protocols

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Poly(ethylene glycol) with a molecular weight of 2000 g/mol (PEG2000) was obtained from Fluka. Poly(ethylene glycol-block-caprolactone) (PEG-CL; AK073; 70411STR-B) and poly(ethylene glycol-blockpolylactide) (PEG-LA; AK009; 190813RAI-B) were obtained from Polyscitech and used for BSA ITC and CD experiments. Poly(ethylene glycol-block-caprolactone) (PEG-CL; AK073; 40319SMS-A) and poly(ethylene glycol-block-polylactide) (PEG-LA; AK009; 180329YSK-A) were obtained from Polyscitech and used for protein fluorescence and HSA ITC and CD experiments. 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (PEG-DSPE) was obtained from Avanti Polar Lipids. Fatty acid-free bovine serum albumin (BSA) was obtained from Millipore Sigma. Fatty acid-free human serum albumin and pyrene were obtained from Sigma-Aldrich. Acetone, sodium chloride, sodium phosphate dibasic anhydrous, sodium phosphate monobasic, 3-(4-morpholino)propane sulfonic acid (MOPS), 4-(2-hydroxyethyl)-1piperazineethanesulfonic acid (HEPES), and sodium dodecyl sulfate (SDS) were obtained from Fisher Scientific. Regenerated cellulose dialysis membranes with a molecular weight cutoff of 3500 g/mol were obtained from Spectrum Laboratories.
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