Fluorescein isothiocyanate affinipure goat anti rabbit igg h l secondary antibodies

Crystal violet, doxycycline hydrochloride, and 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sangon Biotech (Shanghai, China). Matrigel and transwell chambers were purchased from BD Biosciences (San Jose, CA, USA). Propidium iodide (PI) was purchased from Beyotime Biotech (Jiangsu, China). The antibodies to MMP-2, MMP-9, vimentin, and E-cadherin were purchased from Affinity Bioreagents (Colorado, USA). Rabbit polyclonal anti-E-cadherin and rabbit polyclonal anti-MMP-9 were purchased from ZSGB-BIO (Beijing, China). Fluorescein isothiocyanate AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibodies were purchased from EarthOx (San Francisco, USA). Dual luminescence assay kit was purchased from GeneCopoeia (Guangzhou, China).

Apigenin was purchased from Aladdin (Los Angeles, USA). Crystal violet and 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sangon Biotech (Shanghai, China). Matrigel and transwell chambers were purchased from BD Biosciences (San Jose, CA, USA). The antibodies for claudin, N-cadherin, vimentin, and E-cadherin were purchased from Affinity Bioreagents (Colorado, USA). An immunofluorescence staining kit with FITC- and TRITC-labeled goat anti-rabbit IgG and cytoskeleton red fluorescent probe ActinRed were purchased from KeyGEN BioTECH (Nanjing, China). Fluorescein isothiocyanate AffiniPure Goat Anti-Rabbit IgG (H + L) secondary antibodies were purchased from EarthOx (San Francisco, CA, USA). A dual luminescence assay kit was purchased from GeneCopoeia (Guangzhou, China).