The esophageal epithelial cell line (human telomerase reverse transcriptase-immortalized EPC2 cell line) was a kind gift from Dr. Anil Rustgi (University of Pennsylvania, Philadelphia, PA).42 (link)-44 (link) EPC2s were cultured in Keratinocyte Serum-Free media (Life Technologies) supplemented with bovine pituitary extract, epidermal growth factor, and amphotericin (Life Technologies). For differentiated EPC2 cell cultures, EPC2s were grown to confluence for 2 days while being fully submerged in low-calcium ([Ca2+] = 0.09 mM) supplemented Keratinocyte Serum-Free media on 0.4 μm pore-size permeable supports (Corning Incorporated, Corning, NY). Confluent monolayers were then switched to high-calcium ([Ca2+] = 1.8 mM) media for an additional 5 days. The culture medium was removed from the inner chamber of the permeable support in order to expose the cell monolayer to the air interface at day 7. Differentiated EPC2s were analyzed 7 days after ALI induction.
0.4 μm pore size permeable supports
Manufactured by Corning
The 0.4 μm pore-size permeable supports are a laboratory equipment product designed to provide a controlled, permeable barrier for various applications. The core function of these supports is to allow the passage of specific materials while retaining others, based on the 0.4 μm pore size. This product is intended to serve as a versatile tool for researchers and scientists in their laboratory experiments and procedures.
Automatically generated - may contain errors
2 protocols using 0.4 μm pore size permeable supports
1
Differentiation of Immortalized Esophageal Epithelial Cells
2
Differentiation of Immortalized Esophageal Epithelial Cells
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The esophageal epithelial cell line (human telomerase reverse transcriptase-immortalized EPC2 cell line) was a kind gift from Dr. Anil Rustgi (University of Pennsylvania, Philadelphia, PA).42 (link)-44 (link) EPC2s were cultured in Keratinocyte Serum-Free media (Life Technologies) supplemented with bovine pituitary extract, epidermal growth factor, and amphotericin (Life Technologies). For differentiated EPC2 cell cultures, EPC2s were grown to confluence for 2 days while being fully submerged in low-calcium ([Ca2+] = 0.09 mM) supplemented Keratinocyte Serum-Free media on 0.4 μm pore-size permeable supports (Corning Incorporated, Corning, NY). Confluent monolayers were then switched to high-calcium ([Ca2+] = 1.8 mM) media for an additional 5 days. The culture medium was removed from the inner chamber of the permeable support in order to expose the cell monolayer to the air interface at day 7. Differentiated EPC2s were analyzed 7 days after ALI induction.
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