Pad block it

Manufactured by Thermo Fisher Scientific

PAD-BLOCK-iT is a lab equipment product from Thermo Fisher Scientific. It is designed to serve as a blocking agent for reducing non-specific binding in various biochemical assays. The core function of PAD-BLOCK-iT is to minimize background signals and improve the specificity of target detection.

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Lab products found in correlation

Hek293a cells, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

BLOCK-iT (27 citations) PAd/BLOCK-iT™-DEST vector (7 citations) PAD/Block-it system (7 citations) PAd/BLOCK-iT-DEST (5 citations)

2 protocols using pad block it

Adenoviral Knockdown of Ubxd8 Gene

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Oligonucleotides targeting 3 different regions of Ubxd8 were cloned into pTER+/pENTR (Table II in the online-only Data Supplement), which had been modified by addition of a CMV-GFP cassette. The resulting pTER+/pENTR-GFP-mUbxd8^shRNA or pTER+/pENTR-GFP-Scrambled^shRNA were recombined into pAD-BLOCK-iT (Invitrogen), and adenoviral particles were generated and amplified as reported.^{22 (link)}

Loregger A., Raaben M., Tan J., Scheij S., Moeton M., van den Berg M., Gelberg-Etel H., Stickel E., Roitelman J., Brummelkamp T, & Zelcer N. (2017). Haploid Mammalian Genetic Screen Identifies UBXD8 as a Key Determinant of HMGCR Degradation and Cholesterol Biosynthesis. Arteriosclerosis, Thrombosis, and Vascular Biology, 37(11), 2064-2074.

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Cloning and Virus Production of Mouse Erg

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Full-length mouse Erg was cloned into pEC3.1 which contains a CMV promoter and an IRES-Gfp flanked by attL sites. This vector was then recombined using the Gateway Cloning system into pAdBlockIt (Invitrogen). Virus was produced in HEK 293A cells (Invitrogen) following the manufacturer’s protocol. Virus was concentrated by pelleting the cells and resuspending in 1 ml of media before freeze thawing according to the manufacturer’s protocol. Virus was titered by infecting 293A cells with serial dilutions of viral supernatant. After 48 hours, the number of GFP-positive cells was counted and concentration calculated based on dilution. Control virus contained an additional Gfp in place of Erg.

Cox M.K., Appelboom B.L., Ban G.I, & Serra R. (2014). Erg cooperates with TGF-β to control mesenchymal differentiation. Experimental cell research, 328(2), 410-418.

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