Mk 571 sodium salt hydrate

Manufactured by Merck Group

Sourced in United States

MK-571 sodium salt hydrate is a laboratory reagent used in research. It is a white crystalline solid that is soluble in water. The primary function of MK-571 sodium salt hydrate is as a leukotriene receptor antagonist, which can be used in various in vitro and in vivo experiments studying cellular responses and signal transduction pathways.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using mk 571 sodium salt hydrate

Measuring Renal Tubuloid MRP2 Transport

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine MRP2 transmembrane transport functionality of 2D‐cultured renal tubuloids, 5(6)‐Carboxy‐2′,7′‐dichlorofluorescein diacetate (CDFDA; Sigma) was used as a specific fluorescent substrate, and MK571 sodium salt hydrate (MK571; Sigma) was used as a competitive inhibitor for MRP2. Before beginning these transport assays, two important steps were carried out: (1) HBSS buffer was pre‐warmed. (2) The 2D‐cultured inserts were washed carefully with pre‐warmed HBSS buffer to remove all the existing media. After CDFDA and MK571 were dissolved in pre‐warmed HBSS buffer to acquire their working concentration, HBSS containing 10 μM CDFDA was added to the basolateral side of the renal tubuloids in the absence or presence of 20 μM MK571 in the apical and basolateral compartments. After 60 min at 37°C in the dark, 50 μl samples were taken from apical and basolateral compartments, and fluorescence measured at 485/538 nm. Next, MRP2‐specific transport was calculated as the difference in CDFDA absorbance between the non MK571‐inhibited and MK571‐inhibited values.

Nguyen V.V., Ye S., Gkouzioti V., van Wolferen M.E., Yengej F.Y., Melkert D., Siti S., de Jong B., Besseling P.J., Spee B., van der Laan L.J., Horland R., Verhaar M.C, & van Balkom B.W. (2022). A human kidney and liver organoid‐based multi‐organ‐on‐a‐chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell‐derived extracellular vesicles. Journal of Extracellular Vesicles, 11(11), 12280.

+ Open protocol

+ Expand

Cellular Transport Assay Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

We obtained 5(6)‐Carboxy‐2′,7′‐dichlorofluorescein (CDF, a precursor of MRP substrate), MK571 sodium salt hydrate (an MRP inhibitor), Rhodamine 123 (R123, a P‐gp substrate), verapamil (a P‐gp inhibitor), Hoechst 33342 hydrochloride (H33342, a BCRP substrate), and Ko‐143 (a BCRP inhibitor) from Sigma‐Aldrich.

Luo J., Yuan J., Yang Y., Jiang Y., Yan J, & Tong Q. (2023). Special AT‐rich sequence binding protein 1 promotes multidrug resistance in gastric cancer by regulation of Ezrin to alter subcellular localization of ATP‐binding cassette transporters. Cancer Science, 114(4), 1353-1364.

+ Open protocol

+ Expand

Fluorescent Probes for Biological Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sulforhodamine 101 (Sulfo101) was purchased from Chemodex (St. Gallen, Switzerland), 8-(2-[fluoresceinyl]aminoethylthio)adenosine-3′,5′-cyclic monophosphate (8-fluo-cAMP) was purchased from Biolog Life Science (Bremen, Germany), a fluorescent cyclosporine A derivative [N-ϵ(4-nitrobenzofurazan-7-yl)-D-Lys⁸]cyclosporine A (NBD-CsA) was synthesized as previously described (18 (link)), FA-polyethylene glycol PEG-2kDa-FITC was purchased from Biochempeg (Watertown, MA), and NHS-PEG₅-Mal and NHS-PEG₄₀-Mal were obtained from NOF (Tokyo, Japan). SAMSA fluorescein and NHS-TRITC were purchased from Thermo Fisher Scientific (Waltham, MA). Agarose, probenecid, erythromycin, verapamil hydrochloride, p-aminohippuric acid, MK-571 sodium salt hydrate, zosuquidar hydrochloride, saquinavir, indinavir sulfate salt hydrate, folic acid, fluorescein sodium salt (Fluo), FITC-dextran (40, 70, or 150 kDa), 1-phenyl-2-thiourea (PTU), and ethyl-3-aminobenzoate methanesulfonate (MS-222, tricaine) were purchased from Sigma-Aldrich (Buchs, Switzerland).

Bolten J.S., Pratsinis A., Alter C.L., Fricker G, & Huwyler J. (2022). Zebrafish (Danio rerio) larva as an in vivo vertebrate model to study renal function. American Journal of Physiology - Renal Physiology, 322(3), F280-F294.

+ Open protocol

+ Expand

Bovine Lactoferrin Formulation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bovine lactoferrin (bLf), derived from bovine colostrum, was provided as a generous donation by Fonterra, located in Palmerston North, New Zealand. Stearic acid (Grade I, with a purity of at least 99% according to gas chromatography) and a combination of high- and low-methoxyl pectin sourced from apples were used, as well as 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), fluorescein isothiocyanate (FITC), fluorescein sodium salt, sodium azide, verapamil, MK-571 sodium salt hydrate, and ethylenediaminetetraacetic acid (EDTA), acquired from Sigma Aldrich, based in St. Louis, MO, USA. Soybean lecithin was obtained from BDH, located in Poole, UK. Poloxamer 188 was procured from BASF in Ludwigshafen, Germany. Chitosan with low viscosity was purchased from Fluka, situated in Park Rabin Rehovot, Israel. Dulbecco’s Modified Eagles’ Medium (DMEM), fetal calf serum, penicillin-streptomycin-glutamine, nonessential amino acids, trypsin-EDTA, sterile phosphate-buffered saline (PBS) (pH 7.4), and Hank’s balanced salt solution (HBSS) buffer (pH 7.4) were purchased from Life Technologies (Carlsbad, CA, USA). The 4′6-diamindino-2-phenylindole (DAPI) was purchased from Invitrogen (Auckland, New Zealand). The Caco-2 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). All other reagents and chemicals were of analytical grade.

Yao X., Bunt C., Liu M., Quek S.Y., Shaw J., Cornish J, & Wen J. (2023). Enhanced Cellular Uptake and Transport of Bovine Lactoferrin Using Pectin- and Chitosan-Modified Solid Lipid Nanoparticles. Pharmaceutics, 15(8), 2168.

+ Open protocol

+ Expand

Fluorescent Uptake Assay for Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Albumin–fluorescein isothiocyanate conjugate (A9771), Valspodar (PSC833) (SML0572), and MK-571 sodium salt hydrate (M7571) from Sigma-Aldrich, US. Calcein-AM and Cell Tracker Green CMFDA (Life Technologies, U.S.A.).

Ross E.J., Gordon E.R., Sothers H., Darji R., Baron O., Haithcock D., Prabhakarpandian B., Pant K., Myers R.M., Cooper S.J, & Cox N.J. (2021). Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profiles. Scientific Reports, 11, 14053.

+ Open protocol

+ Expand

Fluorescent Probes for Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

2–2 Zn(II) ATP Probe (gift from Dr. Itaru Hamachi, Kyoto, Japan); Vybrant® FAM Caspase-3 and -7 assay kit (V35118), Eosin-5-maleimide (E118), 1,2,3-dihydrorhodamine (D24806), Hank’s balanced salt solution with Ca²⁺ and Mg²⁺ (HBSS⁺⁺) (14025) or without Ca²⁺ and Mg²⁺ (HBSS^—) (14175), YO-PRO^®-1 iodide (λ ex/em 491/509) (Y3603), NuPAGE LDS Sample Buffer (NP0007, Life Technologies, Carlsbad, CA, USA); IgG and protease-free BSA (Jackson ImmunoResearch, West Grove, PA, USA); ATP (A2383-5g), L-glutathione, reduced (G4251), Luperox^® TBH70X tert-butyl hydroperoxide solution (458139), Diphenyleneiodonium chloride (DPI, D2926), sodium orthovanadate (S6508), MK-571 sodium salt hydrate (M7571), ovalbumin (A5503, Sigma-Aldrich); ENLITEN^® ATP Assay System (FF2021), rATP (P1138, Promega, Madison, WI, USA); NSC23788 (553502), glutathione monoethyl ester (353905), GlyH-101 (219671, EMD Millipore, Billerica, MA, USA); 8-bromo cAMP sodium salt (1140), calphostin C (1626), indomethacin (1708), iso-PPADS (0683), KT5720 (1288), Phorbol 12-myristate 13-acetate (PMA) (1201), PPADS (0625), Procaspase-activating compound (PAC) 1 (2581), suramin hexasodium salt (1472), Z-DEVD-FMK (2166, R&D Systems, Minneapolis, MN, USA).

Khoory J., Estanislau J., Elkhal A., Lazaar A., Melhorn M.I., Brodsky A., Illigens B., Hamachi I., Kurishita Y., Ivanov A.R., Shevkoplyas S., Shapiro N.I, & Ghiran I.C. (2016). Ligation of Glycophorin A Generates Reactive Oxygen Species Leading to Decreased Red Blood Cell Function. PLoS ONE, 11(1), e0141206.

+ Open protocol

+ Expand

Oxidative Stress Biomarkers Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

1-chloro-2,4-dinitrobenzene (CDNB), MK571 sodium salt hydrate, glutathione (GSH), 5,5 0 -dithiobis (2-nitrobenzoic acid) (DTNB), Dithiothreitol, Bovine serum albumin, Nicotinamide adenine dinucleotide phosphate (NADPH), Neutral red, Trypan blue, 2 0 ,7 0 -dichlorofluorescein diacetate (DCFDA) and butylhydroxytoluene were purchased from Sigma (USA). Tris (hydroxymethyl) aminomethane (Tris) was purchased from Serva (Germany). 5-Sulfosalicylic acid was purchased from Mallinckrodt Chemicals (Ireland). Folin Ciocalteu reagent was purchased from Biopack (Argentina). Ethylenediaminetetraacetic acid (EDTA) and acetic acid were purchased from Cicarelli (Argentina). glutathione disulfide (GSSG) was purchased from Santa Cruz Biotechnology (USA). Trichloroacetic acid and Thiobarbituric acid were purchased from Merck (Germany). Dimethyl sulfoxide (DMSO) was purchased from Anedra (Argentina). Sodium citrate (Na 3 C 6 H 5 O 7 ) was purchased from Stanton (Argentina). Lysing matrix D was purchased from MP Biomedicals (USA).

Painefilú J.C., Bianchi V.A., Krock B., De Anna J.S., Kristoff G, & Luquet C.M. (2020). Effects of paralytic shellfish toxins on the middle intestine of Oncorhynchus mykiss: Glutathione metabolism, oxidative status, lysosomal function and ATP-binding cassette class C (ABCC) proteins activity. Ecotoxicology and environmental safety, 204.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!