The SC content in the nanoemulsions was assayed by LC after sample dilution in methanol. In order to determine the association efficiency, the nanoemulsion ultrafiltration-centrifugation was performed using Ultrafree® MC 10,000 MW (Millipore) membrane for 10 minutes at 15,000 rpm. The amount of SC associated with the nanoemulsions was calculated as the difference between the total and free SC concentrations determined in the nanoemulsion and ultrafiltrate, respectively.
Ultrafree mc 10 000 mw
Manufactured by Merck Group
Sourced in United States
The Ultrafree® MC 10,000 MW is a laboratory filtration device designed for the rapid separation and concentration of macromolecules, such as proteins and DNA, with a molecular weight cutoff of 10,000 Daltons. The product features a regenerated cellulose membrane that allows for efficient sample recovery and reduced sample loss.
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Lab products found in correlation
4 protocols using ultrafree mc 10 000 mw
1
Quantifying Cannabinoid Encapsulation
2
Encapsulation Efficiency Analysis of Dispersions
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Drug content was analyzed following a slightly modified version of the method described by Fontana et al. [26] , dissolving 1 ml of each dispersion or 1 g of each HG in 25 ml acetonitrile instead of methanol. To determine the encapsulation efficiency, 1 ml of each dispersion was centrifuged (Ultrafree-MC 10,000 MW, Millipore) at 12,000 rpm for 5 min. Next the ultrafiltrate was analyzed for CP content and the drug entrapped was determined by calculating the difference between the total drug and the ultrafiltrate concentrations. The encapsulation efficiency was then obtained by dividing the measured drug entrapped by the total drug content. All measurements were made in triplicate and samples were analyzed by means of HPLC [26] .
3
Quantification and Encapsulation of Diflunisal in Nanocapsules
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Loading rate was determined after dissolution of an aliquot (200 µl) of nanocapsules suspension in 10 ml of mobile phase followed by ultrasound for 10 min and quantified by liquid chromatography (LC) using a previously validated methodology [21] . The chromatographic system consisted of a Gemini RP-18 column (250 mm x 4.6 mm, 5 µm, Phenomenex Torrance, USA) and Shimadzu instrument (LC-10AVP Pump, UV-vis SDP-10AVP, Japan). The mobile phase (at a flow rate of 1.0 ml/min) was composed of acetonitrile/water (80:20%, v/v), the volume of injection was 20 µl, and DFZ was detected at 244 nm. The method was linear (r² = 0.9999) in the range of 5-40 µg/ml, accurate (recovery: 95.75 ± 0.5 %), and precise (R.S.D.: < 1.41% for repeatability and < 2.37 for intermediate precision). Specificity was tested in the presence of the suspension adjuvants, when these factors were shown not to alter the DFZ assay.
The encapsulation efficiency (%) was determined by the difference between total drug (loading rate) and free DFZ concentration. Free DFZ (non-associated to nanostructures) was determined in the ultrafiltrate after separation of the nanoparticles by ultrafiltration/centrifugation (Ultrafree-MC10.000 MW, Millipore, UDA), at 1000 rcf for 10 min. All analysis were performed in triplicate.
The encapsulation efficiency (%) was determined by the difference between total drug (loading rate) and free DFZ concentration. Free DFZ (non-associated to nanostructures) was determined in the ultrafiltrate after separation of the nanoparticles by ultrafiltration/centrifugation (Ultrafree-MC10.000 MW, Millipore, UDA), at 1000 rcf for 10 min. All analysis were performed in triplicate.
4
Quantification of Creatine in Nanoliposomes
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The concentration of creatine in nanoliposomal suspension was measured using a Shimadzu P r o m i n e n c e a p p a r a t u s w i t h U V-Vi s spectrophotometric detection and Phenomenex C18 column (15 x 4.6 mm, 5µm). The mobile phase was methanol-water (50:50, v/v), injection volume was 20 µL and the column temperature was maintained at 25 ºC. The analysis was performed at 0.8 mL/min flow rate with UV detector at 210 nm. The standard curve range was (6.25 -100 µg/mL), quantification and detection limits were 3.39 and 1.13 μg/mL respectively. The encapsulation efficiency (E.E.) was measured by the ultrafiltration/centrifugation method (Ultrafree-MC ® 10,000 MW, Millipore, Bedford, USA). E.E. was calculated as follows: E.E. = (total drug -free drug)/total drug ×100.
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