RNA-seq of MAIT subsets was performed as described previously (5 ). Briefly, for each condition, 200 cells were collected at 4°C in 8 μl of lysis buffer (0.2% Triton X-100, 2 U/μl rRNase inhibitor [Clontech Laboratories/Takara Bio], 5 mM dNTP mix [Life Technologies]) in a 96-well PCR plate (Bio-Rad Laboratories). Immediately after sorting, plates were spun for 1 min at 3000 rpm and stored at −80°C until RNA extraction. Four microliters of each sample were amplified following the Smart-seq2 protocol as described above.
Rrnase inhibitor
Manufactured by Takara Bio
RNase inhibitor is a laboratory reagent used to prevent the degradation of ribonucleic acid (RNA) by RNase enzymes. It maintains the integrity of RNA samples by inhibiting RNase activity during RNA extraction, purification, and other RNA-based procedures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rrnase inhibitor
1
Single-cell RNA-seq of MAIT cell subsets
2
Single-cell RNA-seq of MAIT cell subsets
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