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Hbss 1640

Manufactured by Thermo Fisher Scientific
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Sourced in Slovakia

HBSS 1640 is a sterile, cell culture medium designed for the maintenance and growth of a variety of cell types. It is a balanced salt solution that provides an optimal environment for cell culture, supporting cell adherence and proliferation. The medium contains essential amino acids, vitamins, and other components required for cell growth and survival. HBSS 1640 is a widely used and versatile cell culture medium suitable for various applications in life science research and development.

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Lab products found in correlation

Opti mem 1, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Mg132, by Bio-Techne (2 mentions) Protein g sepharose 4b beads, by Thermo Fisher Scientific (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions) Fluoro carbonyl cyanide phenylhydrazone (fccp), by Bio-Techne (2 mentions) Dc protein assay reagent, by Bio-Rad (1 mentions) Glutamax 1, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Horse serum, by Thermo Fisher Scientific (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Collagen 4, by Merck Group (1 mentions) Neurobasal a, by Thermo Fisher Scientific (1 mentions) Pierce lane marker reducing sample buffer, by Thermo Fisher Scientific (1 mentions) 35 mm glass bottomed dishes, by MatTek (1 mentions) Cellview dishes, by Greiner (1 mentions) Nativepage novex bis tris gel system, by Thermo Fisher Scientific (1 mentions)

2 protocols using hbss 1640

1

Optimization of Cell Culture Reagents

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Reagents were from the following manufacturers: 35‐mm glass‐bottomed dishes (MatTek, P35G‐1.0‐14‐C), CELLview™ dishes (Greiner Bio‐One 627 871), poly‐L‐lysine (Sigma‐Aldrich, P6282), collagen IV (Sigma‐Aldrich, C5533), penicillin, streptomycin and amphotericin B (Invitrogen, 15240‐096), gentamicin (Krka, d. d., Novo Mesto, Slovakia), RPMI‐1640 (Life Technologies, A1049101), DMEM (Life Technologies, 31966021), Neurobasal™ A (Life Technologies, 10888022 and 12349015 (without phenol red)), HBSS 1640 (Life Technologies, 14025050), BME (Life Technologies 41010‐109), Opti‐MEM I (Life Technologies, 31985‐047), B‐27® Supplement (50X, serum free, Life Technologies, 17504044), foetal bovine serum (Life Technologies, 10270106), horse serum (Life Technologies, 26050088), GlutaMAX™‐I (Life Technologies, 3505038) Lipofectamine® 2000 (Invitrogen, 11668‐019), Metafectene® (Biontex Laboratories GmbH, T040), DMSO (Sigma‐Aldrich, 472301), FCCP (Tocris, 0453), G418 (Sigma‐Aldrich, A1720), MG132 (Tocris, 1748), protease inhibitor cocktail cOmplete (Roche, 04693116001), protein G‐sepharose 4B beads (Invitrogen, 101243), DC Protein Assay reagent (Bio‐Rad, 500‐0111), Pierce Lane Marker Reducing sample buffer (Thermo Fisher Scientific, 39000).
Safiulina D., Kuum M., Choubey V., Gogichaishvili N., Liiv J., Hickey M.A., Cagalinec M., Mandel M., Zeb A., Liiv M, & Kaasik A. (2018). Miro proteins prime mitochondria for Parkin translocation and mitophagy. The EMBO Journal, 38(2), e99384.
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2

Mitochondrial Isolation and Analysis

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Reagents were from the following manufacturers: 35-mm glass-bottomed dishes (MatTek, P35G-1.0-14-C), penicillin, streptomycin, and amphotericin B (Invitrogen, 15240-096), RPMI-1640 (Life Technologies, A10491-01), DMEM (Life Technologies, 31966-021), HBSS 1640 (Life Technologies, 14025050), Opti-MEM I (Life Technologies, 31985-047), Lipofectamine 2000 (Invitrogen, 11668-019), Metafectine (Biontex Laboratories GmbH, T040), DMSO (Sigma, 472301), CCCP (Tocris, 0452), FCCP (Tocris, 0453), G418 (Sigma, A1720), MG132 (Tocris, 1748), mitochondrial isolation kit (Mitoscience, MS852/853), Protease inhibitor cocktail, cOmplete (Roche, 04693116001) protein G-sepharose 4B beads (Invitrogen, 101243), DC Protein Assay reagent (Bio-Rad, 500-0111). The NativePAGE™ Novex® Bis-Tris Gel System was from Invitrogen.
Choubey V., Cagalinec M., Liiv J., Safiulina D., Hickey M.A., Kuum M., Liiv M., Anwar T., Eskelinen E.L, & Kaasik A. (2014). BECN1 is involved in the initiation of mitophagy: It facilitates PARK2 translocation to mitochondria. Autophagy, 10(6), 1105-1119.
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