Sb203580 p38 mapk inhibitor

Manufactured by Cayman Chemical

SB203580 is a potent and selective inhibitor of the p38 mitogen-activated protein kinase (MAPK) enzyme. It functions by blocking the activation and catalytic activity of p38 MAPK, which plays a crucial role in various cellular processes.

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Lab products found in correlation

Dylight 800 conjugated goat anti rabbit igg antibody h l, by Thermo Fisher Scientific (2 mentions) Hyclone dmem culture media with and without phenol red, by Thermo Fisher Scientific (2 mentions) Dylight 680 conjugated goat anti rabbit igg antibody h l, by Thermo Fisher Scientific (2 mentions) Alexa 594 donkey anti rabbit secondary, by Jackson ImmunoResearch (2 mentions) Tov112d, by American Type Culture Collection (2 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) 2 methoxyestradiol, by Merck Group (2 mentions) E z n a plasmid maxi kit, by Omega Bio-Tek (1 mentions) Psuper scramble, by Addgene (1 mentions) Jetpei, by Polyplus Transfection (1 mentions) Deadend fluorometric tunel system kit, by Promega (1 mentions) Celltitre 96 aqueous one solution cell proliferation assay kit, by Promega (1 mentions)

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SB203580 (60 citations)

2 protocols using sb203580 p38 mapk inhibitor

Evaluating PKCδ Signaling in Cell Lines

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BG1 cells were obtained from the laboratory of Dr. Ken Korach at NIEHS and Hey C2 cell line was obtained from Dr. Jean Hurteau at Northshore University Health, Evanston Hospital. TOV112D (CRL11731) cell line was purchased from ATCC. HyClone DMEM culture media (with and without phenol red) from ThermoFisher (SH30604.02); 2-methoxyestradiol from Sigma-Aldrich (M6383); 100× HALT protease and phosphatase inhibitor cocktail from ThermoFisher (78440); DyLight™800 conjugated goat anti-rabbit IgG antibody (H&L) (35571) and DyLight™680 conjugated goat anti-rabbit IgG antibody (H&L) (35518) from Thermofisher. Alexa-594 donkey anti-rabbit secondary (133200) from Jackson Immuno Research. jet-PEI^® purchased from Polyplus transfection (catalog no.101-10). Expression plasmids encoding PKCδ^WT (Addgene plasmid # 16386), PKCδ^DN (Addgene plasmid # 16389), and PKCδ^CAT (Addgene plasmid # 16388) were gifts from Bernard Weinstein [74 (link)], pSUPER-PKCδ^RNAi (Addgene plasmid # 10819) was a gift from Alex Toker [75 (link)] and pSUPER-scramble (Addgene plasmid # 118349) was a gift from Lea Sistonen [76 (link)]. E. Z. N. A.^® plasmid maxi kit (D6922-02) was purchased from Omega Bio-Tek. SB203580 p38 MAPK inhibitor was purchased from Cayman Chemical (13067).

Pal P., Hales K, & Hales D.B. (2020). The pro-apoptotic actions of 2-methoxyestradiol against ovarian cancer involve catalytic activation of PKCδ signaling. Oncotarget, 11(40), 3646-3659.

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Comparative Analysis of Cell Line Responses

Cited 1 time

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The HeyC2 cell line [62 (link)] was obtained from Dr. Jean Hurteau at Northshore University Health-Evanston Hospital; TOV112D (CRL11731) cell line was purchased from ATCC. BG1 cells were obtained from Dr. Ken Korach’s lab at NIEHS [63 (link), 64 (link)]. HyClone DMEM culture media (with and without phenol red) from ThermoFisher (SH30604.02); 2-methoxyestradiol from Sigma-Aldrich (M6383); SB203580 p38-MAPK inhibitor from Cayman chemical (13067); DHA (3687) from Tocris Biosciences; 100x HALT protease and phosphatase inhibitor cocktail from ThermoFisher (78440); DyLight™800 conjugated goat anti-rabbit IgG antibody (H&L) (35571) and DyLight™680 conjugated goat anti-rabbit IgG antibody (H&L) (35518) from Thermofisher. Alexa-594 donkey anti-rabbit secondary (133200) from Jackson Immuno Research; DeadEnd Fluorometric TUNEL system kit (G3250) and CellTitre 96® Aqueous one solution cell proliferation assay kit (G3582) were both purchased from Promega (Madison, WI, USA).

Pal P., Hales K., Petrik J, & Hales D.B. (2019). Pro-apoptotic and anti-angiogenic actions of 2-methoxyestradiol and docosahexaenoic acid, the biologically derived active compounds from flaxseed diet, in preventing ovarian cancer. Journal of Ovarian Research, 12, 49.

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