Phospho specific p38 map kinase antibodies

TRAP (H-Ser-Phe-Leu-Leu-Arg-Asn-Pro-Asn-Asp-Lys-Tyr-Glu-Pro-Phe-OH trifluoroacetate salt) was purchased from Bachem AG (Budendorf, Switzerland). Phospho-specific HSP27 (Ser-78) antibodies were purchased from Stressgen Biotechnologies (Victoria, BC, Canada). Phospho-specific Akt (Ser-473 and Thr-308) antibodies, phospho-specific p44/p42 MAP kinase antibodies, phospho-specific p38 MAP kinase antibodies and phospho-specific HSP27 (Ser-82) antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). GAPDH antibodies and deguelin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The PDGF-AB ELISA kit was purchased from R&D (Minneapolis, MN, USA). The phosphorylated-HSP27 ELISA kit was purchased from Enzo Life Science, Inc. (Plymouth Meeting, PA, USA). Other materials and chemicals were obtained from commercial sources. Deguelin was dissolved in dimethyl sulfoxide. The maximum concentration of dimethyl sulfoxide was 0.1%, which did not affect the detection of protein expression by a Western blot analysis.

PDGF-BB was purchased form R&D System, Inc. (Minneapolis, MN, USA). GLP-1 and GIP were obtained from Peptide Institute, Inc. (Osaka, Japan). 8-Bromo cAMP, PD98059, SP600125 or SB203580 were obtained from Calbiochem-Novabiochem Co. (La Jolla, CA, USA). Dibutyryl (Bt2) cAMP was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). H89 was obtained from Seikagaku Co. (Tokyo, Japan). Phospho-specific cAMP response element-binding protein (CREB) antibodies and phospho-specific p38 MAP kinase antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies, anti-osteocalcin mouse antibody and anti-Rho-A (26C4) mouse monoclonal antibody were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). An ECL Western blotting detection system was obtained from GE Healthcare Life Sciences (Chalfront, UK). Exendin-4 was obtained from Abcam (Cambridge, UK). Other materials and chemicals were obtained from commercial sources. H89, PD98059, SP600125 or SB203580 were dissolved in dimethyl sulfoxide. The maximum concentration of dimethyl sulfoxide was 0.3%, which did not affect the cell migration assay or detection of the protein level using Western blotting^{22 (link)}.