Equal amounts of protein (15 μg) were separated on 10%–12% SDS polyacrylamide gels by the method of Laemmli.58 (link) Proteins were probed with anti-Yap-1 monoclonal antibody (1:10,000) (Cell Signaling Technology, Danvers, MA, USA), anti-BMI-1 (1:10,000) (Cell Signaling Technology), anti-Wee1 (1:500) (Cell Signaling Technology), anti-Chk1 (1:500) (Cell Signaling Technology), anti-Cdc2 (1:500) (Cell Signaling Technology), anti-p27 (1:500) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-Cyclin A (1:500) (Santa Cruz Biotechnology), and anti-GAPDH (1:200,000) (Santa Cruz Biotechnology). Horseradish peroxidase-conjugated antibodies against mouse or rabbit (1:5,000, Santa Cruz Biotechnology) were used as the secondary antibodies. Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system and SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Western blots were quantified using Image Studio software. All western blots were repeated three times for quantification.
Anti yap 1 monoclonal antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-Yap-1 monoclonal antibody is a laboratory reagent used for the detection and analysis of the Yap-1 protein, a transcriptional regulator that plays a role in cellular growth and development. This antibody can be utilized in various research applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the Yap-1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Santa Cruz Biotechnology (2 mentions)
Anti bmi1, by Cell Signaling Technology (2 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase conjugated antibodies against mouse or rabbit, by Santa Cruz Biotechnology (2 mentions)
Anti p27, by Santa Cruz Biotechnology (1 mentions)
Anti wee1, by Cell Signaling Technology (1 mentions)
Anti chk1, by Cell Signaling Technology (1 mentions)
Anti cdc2, by Cell Signaling Technology (1 mentions)
Anti cyclin a, by Santa Cruz Biotechnology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti α tubulin, by Santa Cruz Biotechnology (1 mentions)
Protease inhibitor, by Merck Group (1 mentions)
Anti sox2, by Cell Signaling Technology (1 mentions)
Anti c myc, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti yap 1 monoclonal antibody
1
Western Blot Analysis of Cell Signaling Proteins
2
Western Blot for Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Equal amounts of protein (15 μg) extracted from cells lysed in RIPA buffer with protease inhibitor (Sigma) were separated on 10%-12% sodium dodecyl sulfate-polyacrylamide gels by the method of Laemmli [58 (link)]. Proteins were probed with rabbit anti-YAP1 monoclonal antibody (1:10000) (Cell Signaling Technologies), anti-DLCK1 (1:500) (Abcam), anti-BCL2 (1:500) (NeoMarkers), anti-BMI1 (1:10000) (Cell Signaling Technologies), anti-SOX2 (1:1000) (Cell Signaling Technologies), anti-c-MYC (1:500) (Santa Cruz Biotech Inc.), anti-TS (1:500) (Millipore), anti-α-tubulin (1:50000) (Santa Cruz Biotech Inc.), anti-GAPDH (1:100000) (Santa Cruz Biotech Inc.) and anti-β-actin (1:50000) (Sigma Aldrich). Horseradish peroxidase conjugated antibodies against mouse or rabbit (1:5000, Santa Cruz Biotech Inc.) were used as the secondary antibodies. Protein bands were visualized with autoradiography film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fischer). Western blot density was quantified using Image J software. All Western blots were repeated 3 times for quantification.
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