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Rb anti β actin

Manufactured by Cell Signaling Technology
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Rb anti-β-Actin is a rabbit polyclonal antibody that recognizes β-actin, a widely expressed cytoskeletal protein. It can be used for the detection of β-actin in a variety of applications, including Western blotting, immunoprecipitation, and immunofluorescence.

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Lab products found in correlation

Rb anti total p70 s6k, by Cell Signaling Technology (2 mentions) Goat anti rabbit secondary antibody conjugated to alexa fluor 488, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

β-actin (6244 citations) Anti-β-actin (1878 citations) Anti-actin (219 citations) Anti-Rb (46 citations)

3 protocols using rb anti β actin

1

Protein Signaling Assay for mTORC1/2

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NP-40 extracts of snap frozen spleens or primary adherent or non-adherent splenocytes were separated by SDS-PAGE, transferred to nitrocellulose, and stained with the following antibodies (Cell Signaling Technologies, Beverly, MA) at a 1:1,000 dilution: Rb anti-β-Actin, Rb anti-total p70 S6K, Rb anti-phospho p70 S6K (Thr389), mouse anti-total S6, Rb anti-phospho S6 (Ser235/336 and Ser240/244) for mTORC1 signaling, and with .... for mTORC2 signaling.
Mejia P., Treviño-Villarreal J.H., Hine C., Harputlugil E., Lang S., Calay E., Rogers R., Wirth D., Duraisingh M.T, & Mitchell J.R. (2015). Dietary restriction protects against experimental cerebral malaria via leptin modulation and T cell mTORC1 suppression. Nature communications, 6, 6050.
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2

Antibody Reagents for Immunoblotting and ELISA

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Rabbit (Rb) anti-p65, Rb anti-phospho p44/42 MAPK (ERK1/2) (pTpY202/204), Rb anti-p44/42 MAPK (ERK1/2), Rb anti-phospho-IκBα (Ser32), Rb anti-IκBα, Rb anti-phospho-c-Jun (Ser63), Rb anti-c-Jun and Rb anti-β-actin (horse radish peroxidase (HRP) conjugate) antibodies were all purchased from Cell Signaling Technology (Beverly, MA). HRP-conjugated donkey anti-rabbit secondary antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Goat-anti-rabbit secondary antibody conjugated to Alexa Fluor ® 488 was obtained from Invitrogen. TNF-α, and IL-6 capture antibodies and biotin-conjugated detection antibodies for ELISA were purchased from eBioscience (San Diego, CA).
Malone A., Clark R.F., Hoskin D.W, & Power Coombs M.R. (2022). Regulation of macrophage-associated inflammatory responses by species-specific lactoferricin peptides. Frontiers in bioscience (Landmark edition), 27(2).
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3

Protein Signaling Assay for mTORC1/2

Check if the same lab product or an alternative is used in the 5 most similar protocols
NP-40 extracts of snap frozen spleens or primary adherent or non-adherent splenocytes were separated by SDS-PAGE, transferred to nitrocellulose, and stained with the following antibodies (Cell Signaling Technologies, Beverly, MA) at a 1:1,000 dilution: Rb anti-β-Actin, Rb anti-total p70 S6K, Rb anti-phospho p70 S6K (Thr389), mouse anti-total S6, Rb anti-phospho S6 (Ser235/336 and Ser240/244) for mTORC1 signaling, and with .... for mTORC2 signaling.
Mejia P., Treviño-Villarreal J.H., Hine C., Harputlugil E., Lang S., Calay E., Rogers R., Wirth D., Duraisingh M.T, & Mitchell J.R. (2015). Dietary restriction protects against experimental cerebral malaria via leptin modulation and T cell mTORC1 suppression. Nature communications, 6, 6050.
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