Anti tgf β1 antibody

Male Sprague-Dawley (SD) rats, 6 weeks old (200–230 g), were provided by the experimental animal center of Binzhou Medical University. The experimental animals were treated according to the guidelines approved by university committee on the use and care of laboratory animals. Trizol and the two-step PCR detection kits were purchased from the TaKaRa Co. (Dalian, China). Anti-α-SMA antibodies, anti-TGF-β1 antibody, anti-MMP-1 antibody, and anti-NF-κB p65 antibody for immunocytochemistry were purchased from the Boster Co. (Wuhan, China). The light microscope and camera were purchased from Nikon Corporation (Japan). All other chemicals and reagents were of analytical grade.

C57 mice (weight 20–25 g, male) were obtained from the Changzhou CAVENS Laboratory Animal (Jiangsu, China). HK-2 cells were acquired from the BeNa Culture Collection (Beijing, China). The following materials were also used: anti-FNDC3B antibody (Atlas Antibodies AB, Sweden); anti-TGF-β1 antibody (Boster, Wuhan, China); anti-GAPDH antibody (Service Bio, Wuhan, China); anti-caspase3 antibody (Boster, Wuhan, China); TRIzol (Invitrogen, USA); fetal bovine serum (Gibco, USA); TUNEL Kit (Roche, Shanghai, China); Hoechst (Service Bio, Wuhan, China); Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA); Bio-Rad S1000 with Bestar SYBR Green RT-PCR Master Mix (DBI Bioscience, Shanghai, China); and AAV GPAAV-CMV-MCS-EF1-ZsGreen1-WPRE (Genomeditech Co., LTD, Shanghai, China).