Gene expression of inducible breast cancer cell lines BT549, CAMA-1, HCC1428, Hs578T, MCF7, T47D, and ZR-75-1 was assessed using the NanoString platform (NanoString Technologies, Seattle, WA, USA), which has been described previously [28] (link). Briefly, sample RNA was hybridized with a custom 345-gene codeset (NanoString Technologies, WA, USA) at 65°C for 16 hours. Hybridized probe:target mixture was then purified and quantified via nCounter MAX Digital Analyzer (NanoString Technologies, WA, USA). The custom 345-gene codeset used has been described previously [29] (link) and includes ABCB1 as a profiling target (Suppl. Table 3). Raw counts were normalized to internal positive control probes and housekeeping genes according to default parameters in nSolver version 4.0 (NanoString Technologies, WA, USA), with background threshold set to 20. Normalized counts data are available at https://github.com/jasminerethmeyer/ABCB1 .
345 gene codeset
Manufactured by NanoString
Sourced in United States
The 345-gene codeset is a lab equipment product from NanoString. It is designed to enable the detection and quantification of 345 genes in a single reaction. The codeset provides a targeted approach to gene expression analysis, allowing for the simultaneous measurement of multiple genes within a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 345 gene codeset
1
Breast Cancer Cell Line Gene Expression
2
NanoString nCounter Gene Expression Profiling
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The NanoString nCounter platform gene expression assay has been described previously [20 (link)]. Briefly, the NanoString nCounter platform assays gene expression directly from RNA samples via hybridization of samples with a set of multiplexed nucleotide probes. Probes for each gene target are uniquely barcoded with a series of fluorophores. Fluorescence microscopy imaging of sample-hybridized fluorophore-labeled probes generates quantitative counts data for each gene in each sample.
For gene expression profiling on the nCounter system, patient sample or HMEC control RNA was first hybridized with the custom 345-gene codeset (NanoString Technologies, WA, USA) at 65 °C for 16 h. Post-hybridization probe:target mixture was then purified and quantified via nCounter MAX Digital Analyzer (NanoString Technologies, WA, USA).
For gene expression profiling on the nCounter system, patient sample or HMEC control RNA was first hybridized with the custom 345-gene codeset (NanoString Technologies, WA, USA) at 65 °C for 16 h. Post-hybridization probe:target mixture was then purified and quantified via nCounter MAX Digital Analyzer (NanoString Technologies, WA, USA).
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