Apc anti human cd8 antibody

Antibodies for flow cytometry assays were used for measurement of cell markers, which include FITC anti-mouse/human Ki-67 Antibody (151211, Biolegend, USA), APC anti-human CD8 antibody (980904, Biolegend, USA), PE anti-human CD28 antibody (302907, Biolegend, USA) and PE/Cyanine7 anti-human CD45RO Antibody (304229, Biolegend, USA). Matched immunoglobulin G (IgG) antibodies were used as isotype controls. Flow cytometry assays were performed using according to the manufacturer's instructions. Cell sorting was conducted with a Beckman CytoFLEX S flow cytometer (Beckman) using Becton CytExpert software. Data were analyzed using FlowJo V10 software.

CellTrace™ CFSE cell proliferation kit (Invitrogen) was used to stain activated PBMCs with CFSE prior to co-culture with NPC cells, as per manufacturer’s instructions. After 48-hour co-culture, the proliferation of CD8+ T cells was determined by staining the APC anti-human CD8 antibody (BioLegend), and CFSE intensity in the CD8⁺ subpopulation was measured by NovoCyte Quanteon Flow Cytometer, and proliferation index was quantified by the built-in CFSE analysis module in NovoExpress software.