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Anti hcd3 efluor 605nc

Manufactured by Thermo Fisher Scientific

The Anti-hCD3-eFluor 605NC is a fluorescently labeled antibody for the detection of human CD3 in flow cytometry applications. It is conjugated with the eFluor 605NC fluorescent dye.

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2 protocols using anti hcd3 efluor 605nc

1

Chemokine Receptor Expression on Lymphocytes

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The expression of CCR2, CCR4, CCR5, CCR6, CCR7, and CXCR4 on lymphocytes was analyzed by flow cytometry using anti-hCD3-eFluor 605NC or anti-hCD3-eFluor 450, anti-hCD4-FITC, anti-hCD8-PE-Cy7, anti-hCD25-APC, anti-hCD45RA-eFluor 450, anti-hFoxP3-PE, anti-hCD14-FITC, anti-hCD19-PE-Cy7, anti-hCD11c-Alexa Fluor 700, anti-hCD16-PE, anti-hCD56-APC, anti-hCCR6-PerCP-eFluor710, anti-hCCR7-APC-eFluor780, and anti-hCXCR4-PE-Cy7 antibodies (eBioscience). The anti-hCCR2-Alexa Fluor 647, anti-hCCR4-PerCP-Cy5.5, and anti-hCCR5-APC-Cy7 antibodies were purchased from BD Pharmingen (San Diego, CA, USA).
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2

Phenotyping Tumor-Infiltrating T Cells

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To identify FoxP3+CD4+ T cell subsets, T cells obtained from tumor tissues, adjacent non-tumor tissues, and blood were stained with anti-hCD3-eFluor 605NC, anti-hCD4-FITC, anti-hCD25-APC, anti-hCD45RA-eFluor 450, and anti-hFoxP3-PE antibodies (eBioscience).
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