Spd 10ad vp

Manufactured by Shimadzu

Sourced in Japan

The SPD-10AD VP is a diode array detector for liquid chromatography (LC) systems. It is designed to detect and measure the absorbance of light passing through a liquid sample at multiple wavelengths simultaneously. The device is capable of rapid data acquisition and provides high-sensitivity detection for a wide range of analytical applications.

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Lab products found in correlation

M 560, by Büchi (1 mentions) Xwin nmr software, by Bruker (1 mentions) Irprestige 21 fourier transform infrared ftir spectrophotometer, by Shimadzu (1 mentions) Dpx 300 spectrometer, by Bruker (1 mentions) 8453 uv vis spectrophotometer, by Agilent Technologies (1 mentions) Lc 10at vp, by Shimadzu (1 mentions) Dgu 14a, by Shimadzu (1 mentions) Sil10 advp, by Shimadzu (1 mentions) Gemini c18 reverse phase, by Phenomenex (1 mentions) Class vp hplc system, by Shimadzu (1 mentions) Sil 10ad, by Shimadzu (1 mentions) Lc 10, by Shimadzu (1 mentions)

3 protocols using spd 10ad vp

Comprehensive Analytical Characterization of Novel Compounds

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All chemicals were obtained from Sigma-Aldrich or Merck. NMR Spectra were registered on Bruker DPX 300 spectrometer at room temperature (298K) on a using DMSO-d6 as the solvent and processed using Bruker XWinNMR software. LC/MS was developed by means of chromatography with PHENOMENEX GEMINI NX C18 110Å 4.61 × 150 mm column (0.05% TFA, gradient MeCN/H₂O), UV-detector SHIMADZU SPD-10AD VP (registered absorption at 254 nm), ELSD (evaporative light scattering detector) SEDEX-75 and API-150EX mass-spectrometer. Elution started with 0.1 M solution of TFA in water and ended with 0.1 M solution of TFA in acetonitrile used a linear gradient at a flow rate of 0.15 mL/min and an analysis cycle time of 25 min. FT-IR spectrum was registered in KBr pellet with Shimadzu IR Prestige-21 Fourier Transform Infrared (FTIR) Spectrophotometer. UV/Vis spectrum was registered in acetonitrile with Agilent 8453 UV-Vis Spectrophotometer. Melting point was registered with Buchi M-560. Elemental analysis was performed on EuroEA-3000 CHNS-O Analyzer.

Ivachtchenko A.V., Mitkin O.D., Kravchenko D.V., Kovalenko S.M., Shishkina S.V., Bunyatyan N.D., Konovalova I.S., Dmitrieva I.G., Ivanov V.V, & Langer T. (2019). Synthesis, X-ray crystal structure, Hirshfeld surface analysis, and molecular docking study of novel inhibitor of hepatitis B: methyl 4-fluoro-3-(morpholinosulfonyl)benzo[b]thiophene-2-carboxylate. Heliyon, 5(11), e02738.

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HPLC Quantification of Efavirenz in Nanoparticles

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The amount of the EFV in the nanoparticles and filtrate was analyzed by using a reverse phase-HPLC method described in our earlier paper.^{26 (link)} The HPLC apparatus consisted of a pump (LC-10ATvp), system controller (SIL-10ADvp), degasser unit (DGU-14A), refrigerated auto-sampler (SIL-10ADvp), a UV-Vis detector (SPD-10ADvp) and a column heater (Shimadzu Corporation, Columbia, MD). Samples were run through a C₁₈ pre-column and a Gemini C₁₈ reverse-phase [150 × 4 5 mm (I.D.)] with 5 μm particle size packing (Phenomenex, Torrance, CA). The mobile phase consisted of acetonitrile and 25 mM KH₂PO₄ solution (55:45). For HPLC analysis, the flow rate of the mobile phase was at 0.9 ml/min, column oven was set at 35°C, injection volume was 20 μl and detector was set at 212 nm. The retention time for EFV was 10.4 min. For standard curve, EFV stock solution (1 mg/ml) was prepared in acetonitrile. The stock solutions were diluted with acetonitrile to obtain solutions of various concentrations. Standard curve was obtained by injecting 0.1–10 μg/ml of EFV. Limit of detection for EFV was 50 ng/ml. All the experiments were performed in triplicate. The inter-day and intra-day variability for the standard curve was always < 10%.

Date A.A., Shibata A., McMullen E., La Bruzzo K., Bruck P., Belshan M., Zhou Y, & Destache C.J. (2015). Thermosensitive Gel Containing Cellulose Acetate Phthalate-Efavirenz Combination Nanoparticles for Prevention of HIV-1 Infection. Journal of biomedical nanotechnology, 11(3), 416-427.

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HPLC Analysis of Pharmaceutical Compound

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The analyses were carried out on a Shimadzu Class VPHPLC system (Tokyo, Japan) equipped with SCL 10 A VP system controller, SPD-10 AD VP UV-Visible detector and LC 10 AD VP solvent delivery with SIL-10 AD VP auto injector. The column used was thermo-hypersil C18: 250 mm×4.6 mm i.d., 5 µm end capped column, with Phosphoric acid (2.45 g/L H3PO4), Acetonitrile (87+13, v/v) as a mobile phase at flow rate of 1.5 ml/min AUVdetector with a wavelength of 278 nm and injection volume of 50 µL was applied.

Alaboudi A.R., Almashhadany D.A., Abu‐Basha E., & Musallam I. (2021). Enrofloxacin and Ciprofloxacin Residues in Table Eggs: Distribution and Heat Treatment Effect. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies, 78(1), 10-17.

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