Bladder cancer cell lines 5637, HT1197, HT1376, J82, RT4, SCaBER, SW780, T24, TCCSUP and UM-UC3 were purchased from ATCC (Manassas, VA, USA). 647V, BC-3C, BFTC-905, CAL-29, JMSU-1, KU-19-19, RT-112, SW1710 and VM-CUB1 were purchased from DSMZ (Riverdale, MD, USA). 253J, SV-HUC, TSU-PR1, UM-UC14 and WH were obtained from Dr. Jer-Tsong Hsieh (University of Texas Southwestern, Dallas, TX, USA). Bladder cell lines 94-10, 96-1, 97-1, 97-7, 97-18, 97-24, DSH1, JO’N, SD, VM-CUB2 and VM-CUB3 were obtained from Dr. Margaret A. Knowles (University of Leeds, Leeds, UK). Cell lines were re-authenticated via short tandem repeat (STR) analysis using the Cell-ID-system (G9500, Promega, Nacka, Sweden), and products analyzed using an Applied Biosystems 3130 Genetic Analyzer (San Francisco, CA, USA). Cell lines were cultured according to the manufacturer recommendations. We performed tests for Mycoplasma sp. and cell identification by Single Nucleotide Polymorphism (SNP) using the MycoSEQ detection kit (Applied Biosystems, Waltham, MA, USA).
Bc 3c
Manufactured by Leibniz Institute DSMZ
Sourced in United States, Germany
The BC-3C is a laboratory equipment used for centrifugation. It is a benchtop centrifuge designed to separate various components of a liquid sample based on their density and size. The BC-3C can accommodate sample volumes up to 15 mL and can reach a maximum speed of 3,000 rpm.
Automatically generated - may contain errors
Lab products found in correlation
Umuc3, by American Type Culture Collection (2 mentions)
Tccsup, by American Type Culture Collection (2 mentions)
Sw780, by American Type Culture Collection (2 mentions)
Ku 19 19, by Leibniz Institute DSMZ (2 mentions)
Ht1197, by American Type Culture Collection (2 mentions)
Ht1376, by American Type Culture Collection (2 mentions)
Rt112, by Leibniz Institute DSMZ (2 mentions)
Vm cub1, by Leibniz Institute DSMZ (2 mentions)
Cal 29, by Leibniz Institute DSMZ (2 mentions)
Bftc 905, by Leibniz Institute DSMZ (2 mentions)
Sw 1710, by Leibniz Institute DSMZ (2 mentions)
3130 genetic analyzer, by Thermo Fisher Scientific (1 mentions)
Scaber, by American Type Culture Collection (1 mentions)
Mycoseq detection kit, by Thermo Fisher Scientific (1 mentions)
Cell id system, by Promega (1 mentions)
Jmsu 1, by RIKEN BioResource Center (1 mentions)
Kmbc2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
2 protocols using bc 3c
1
Characterization of Bladder Cancer Cell Lines
2
Establishing Chemotherapy-Resistant Bladder Cancer Cell Lines
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HT‐1197, HT‐1376, J82, RT4, SW 780, TCCSUP, and UM‐UC‐3 were purchased from ATCC (Manassas, VA, USA). 647‐V, BC‐3C, BFTC‐905, CAL‐29, KU‐19‐19, RT‐112, SW‐1710 and VM‐CUB1 were purchased from DSMZ (Braunschweig, Germany). EJ138, U‐BLC1, UM‐UC‐9 and UM‐UC‐14 were purchased from ECACC (Salisbury, UK). KMBC‐2 and T24 were purchased from JCRB Cell Bank (Osaka, Japan). BOY‐12E, and JMSU‐1 were provided by the RIKEN BRC (Tsukuba, Japan). These cell lines were cultured according to the guidelines from the suppliers.
To generate chemotherapy‐resistant cell lines, UM‐UC‐14 and RT‐112 cell lines were exposed to adriamycin and gemcitabine, respectively, whose concentrations were gradually increased up to 100 and 1000 ng/mL, respectively. Adriamycin‐resistant UM‐UC‐14 and gemcitabine‐resistant RT‐112 cell lines were maintained in the culture medium containing 50 ng/mL adriamycin and 1000 ng/mL gemcitabine, respectively.
To generate chemotherapy‐resistant cell lines, UM‐UC‐14 and RT‐112 cell lines were exposed to adriamycin and gemcitabine, respectively, whose concentrations were gradually increased up to 100 and 1000 ng/mL, respectively. Adriamycin‐resistant UM‐UC‐14 and gemcitabine‐resistant RT‐112 cell lines were maintained in the culture medium containing 50 ng/mL adriamycin and 1000 ng/mL gemcitabine, respectively.
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