Mouse anti g3bp1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Mouse anti-G3BP1 is a primary antibody that recognizes the G3BP1 (Ras-GTPase-activating protein SH3-domain-binding protein 1) protein. G3BP1 is involved in stress granule formation and modulates cellular stress response pathways. This antibody can be used for the detection and analysis of G3BP1 in various experimental techniques.

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Lab products found in correlation

Rabbit anti eif2α, by Cell Signaling Technology (2 mentions) Mouse anti puromycin, by Merck Group (2 mentions) Goat anti eif3b, by Santa Cruz Biotechnology (1 mentions) Rabbit anti 4e bp1, by Cell Signaling Technology (1 mentions) Rat anti tubulin, by Abcam (1 mentions) Rabbit anti eif4g, by Cell Signaling Technology (1 mentions) Rabbit anti caprin 1, by Proteintech (1 mentions) Ab51502, by Abcam (1 mentions) Ab23345, by Abcam (1 mentions) Ab86135, by Abcam (1 mentions) Ab70319, by Abcam (1 mentions) Ab31940, by Abcam (1 mentions) Ab18465, by Abcam (1 mentions) Sc 365338, by Santa Cruz Biotechnology (1 mentions) A5441, by Merck Group (1 mentions) H3663, by Merck Group (1 mentions) Mouse anti ha, by Merck Group (1 mentions) Rabbit anti phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Rabbit anti lc3b, by Cell Signaling Technology (1 mentions) Peroxidase coupled secondary antibodies, by Cell Signaling Technology (1 mentions)

3 protocols using mouse anti g3bp1

Molecular Profiling of Translation Regulation

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The following primary antibodies were used in this study: mouse anti-G3BP1 (Santa Cruz, sc-81940, Santa Cruz, CA, USA), goat anti-eIF4AI (Santa Cruz, sc-14211), mouse anti-eIF4E (P-2, Santa Cruz, sc-9976), rabbit anti-eIF4G (Cell Signaling #2498), goat anti-eIF3B (Santa Cruz, sc-16377), rabbit anti-Caprin1 (Proteintech, 15112-1-AP, Chicago, IL, USA), rabbit anti-phospho(S51)-eIF2α (Cell Signaling #9721), rabbit anti-eIF2α (Cell Signaling #9722, Danvers, MA, USA), rabbit anti-4E-BP1 (Cell Signaling, #53H11), rabbit anti-phospho-4E-BP1(Thr37/46) (Cell Signaling, #236B4), rabbit anti-AMPKalpha (Cell Signaling, #2603), rabbit anti-phospho-AMPKalpha (Cell Signaling, #2535), rat anti-tubulin (Abcam, #ab6160, Cambridge, UK), rabbit anti-Trx1 (FL-105, Santa Cruz sc-20146) (WB), rabbit anti-Trx1 (Cell Signaling #2429) (IF) and mouse anti-puromycin (Millipore MABE343).

Eiermann N., Stoecklin G, & Jovanovic B. (2022). Mitochondrial Inhibition by Sodium Azide Induces Assembly of eIF2α Phosphorylation-Independent Stress Granules in Mammalian Cells. International Journal of Molecular Sciences, 23(10), 5600.

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Antibodies for Western Blot and Immunofluorescence

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The antibodies used for Western blot analysis and immunofluorescence included mouse anti-HA (1:2000 for Western blot and 1:800 for immunofluorescence; H3663) from Millipore-Sigma, mouse anti–glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10,000; g9295) from Millipore-Sigma, mouse anti-G3BP1 (1:500 for Western blot and 1:200 for immunofluorescence; sc-365338) from Santa Cruz Biotechnology, rabbit anti-G3BP2 (1:2000; ab86135) from Abcam, rabbit anti-TIA1 (1:300; 12133-2-AP) from Proteintech, rabbit anti-CAPRIN1 (1:2000; 15112-1-AP) from Proteintech, rabbit anti-UBAP2L (1:4000; ab70319) from Abcam, mouse anti-actin (1:10,000; A5441) from Millipore-Sigma, mouse anti-tubulin (1:1000; T8328) from Millipore-Sigma, mouse anti-Satb2 (1:100; ab51502) from Abcam, rat anti-Ctip2 (1:200; ab18465) from Abcam, rabbit anti-Tbr1 (1:200; ab31940) from Abcam, rabbit anti-Pax6 (1:200; 901301) from BioLegend, and rabbit anti-Tbr2 (1:200; ab23345) from Abcam.

Jia X., Zhang S., Tan S., Du B., He M., Qin H., Chen J., Duan X., Luo J., Chen F., Ouyang L., Wang J., Chen G., Yu B., Zhang G., Zhang Z., Lyu Y., Huang Y., Jiao J., Chen J.Y., Swoboda K.J., Agolini E., Novelli A., Leoni C., Zampino G., Cappuccio G., Brunetti-Pierri N., Gerard B., Ginglinger E., Richer J., McMillan H., White-Brown A., Hoekzema K., Bernier R.A., Kurtz-Nelson E.C., Earl R.K., Meddens C., Alders M., Fuchs M., Caumes R., Brunelle P., Smol T., Kuehl R., Day-Salvatore D.L., Monaghan K.G., Morrow M.M., Eichler E.E., Hu Z., Yuan L., Tan J., Xia K., Shen Y, & Guo H. (2022). De novo variants in genes regulating stress granule assembly associate with neurodevelopmental disorders. Science Advances, 8(33), eabo7112.

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Western Blot Analysis of Cellular Stress Markers

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Total cell lysates were harvested in Laemmli lysis buffer and protein concentration determined using the DC Protein Assay (Bio-Rad). Protein lysates were separated in 12% TGX stain-free gels which were then activated for 45 s after SDS-electrophoresis, transferred to PVDF membranes using the Trans-Blot Turbo transfer system and imaged with the ChemiDoc Touch imaging system (Bio-Rad). Primary antibodies were used as follows: mouse anti-puromycin (1:8000; EMD Millipore, MABE343), rabbit anti-eIF2α (1:1000; Cell Signaling Technology, 9722), rabbit anti-phospho-eIF2α (Ser51) (1:500; Cell Signaling Technology, 9721), rabbit anti-ribosomal protein S6 (rpS6) (1:4000; Cell Signaling Technology, 2317), rabbit anti-phospho-rpS6 (Ser235/236) (1:4000; Cell Signaling Technology, 2211), rabbit anti-GADD34 (1:750; Thermo Fisher Scientific, PA1-139), rabbit anti-LC3B (1:1000; Cell Signaling Technology, 2775), mouse anti-SQSTM1/p62 (D5L7G) (1:1000; Cell Signaling Technology, 88588), mouse anti-G3BP1(1:250, Santa Cruz Biotechnology, sc-81940), rabbit anti-G3BP2 (1:2500; Sigma-Aldrich, HPA018304). Detection was performed with peroxidase-coupled secondary antibodies (Cell Signaling Technology) with Clarity Western ECL substrate (Bio-Rad). All blots were normalized to total lane protein and band intensities were quantified using ImageLab software (Bio-Rad).

Attwood K.M., Robichaud A., Westhaver L.P., Castle E.L., Brandman D.M., Balgi A.D., Roberge M., Colp P., Croul S., Kim I., McCormick C., Corcoran J.A, & Weeks A. (2020). Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cells. Cell Death & Disease, 11(11), 989.

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