Anti cytochrome c ab133504

Protoporphyrin IX (PpIX, P8293), zinc protoporphyria (ZnPP, 282820), tetrahydrofuran (THF, 401757), triethylamine (TEA, T0886), N-acetylcysteine (NAC, A7250), nicotinamide adenine dinucleotide phosphate (NADPH, 621706), hemin (51280), and 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI, D8417) were purchased from Sigma (Shanghai, China). Isobutyl chloroformate (01086117) was purchased from Adamas (Shanghai, China). Ethylene diamine (2143) and chloroform (3084) were purchased from Damao (Tianjin, China). The methoxy (polyethylene glycol) succinimidyl carboxyl methyl ester (mPEG-NHS, PS1-H-1K), with an average molecular weight of 1000, was purchased from Ponsure (Shanghai, China). Zinc acetate (Z111245) was purchased from Aladdin (Shanghai, China). Cell Counting Kit-8 (CCK-8,C0039), 2′,7′-dichlorofluorescein diacetate (DCFH-DA, S0033S), and Mito-Tracker Green (C1048) were purchased from Beyotime (Shanghai, China). Anti-PCNA (60097) was purchased from Proteintech (Wuhan, China). Anti-cytochrome c (ab133504) was purchased from Abcam (Shanghai, China), and anti-cleaved caspase-3 (9661) was purchased from Cell Signaling Technology (Shanghai, China).

MCI-186, U0126, PD98059, LY294002, SH-5, NAC, and DPI were purchased from Biomol Research Laboratories Inc. (Plymouth Meeting, PA, United States). Z-VAD-FMK and MitoTEMPOL were purchased from Sigma-Aldrich Corp. (St. Louis, MO, United States). Anti-GAPDH (#SC-32233), anti-Akt (#SC-8312), anti-phospho-Akt (#SC-7985) and anti-COX IV antibodies and APO were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, United States). Anti-caspase-3 (#9662S), anti-caspase-7 (#12827S), anti-caspase-9 (#9508S), anti-Bax (#9239S), anti-Bad (#5023S), anti-Bcl-2 (#15071S) and anti-phospho-ERK1/2 (#SC-8312) antibodies were purchased from Cell Signaling Technology Inc. (Denver, MA, United States). Anti-PARP (#13371-AP) was purchased from Proteintech Group (Manchester, United Kingdom), and anti-cytochrome C (#ab133504) was purchased from Abcam (Cambridge, United Kingdom). Artocarpin was purchased from Pulin Biotech Company Limited (Taipei, Taiwan). Artocarpin purity was determined by high-performance liquid chromatography (HPLC) and found to be >98%.

Parental and Mcl-1 knockout (KO) H1299 cells were maintained in RPMI 1640 medium supplemented with 10% FBS as our previously described (Chen et al. 2018 (link)). Wild type (WT) and Mcl-1 KO MEF (mouse embryonic fibroblast) cells were cultured in DMEM medium supplemented with 10% FBS as previously described (Chen et al. 2018 (link)). Anti-Mcl-1 (#94296), anti-cleaved caspase 3 (#9661) and anti-Bim (#2933) were purchased from Cell Signaling Technology (MA, USA). Anti-Bcl-2 (sc-7382), anti-Bax (sc-7480), anti-PARP1 (sc-8007) and β-Actin (sc-8432) were obtained from Santa Cruz Biotechnology (CA, USA). Anti-Cytochrome C (ab133504), Anti-Bcl-xL (ab32370) and Anti-Bak (ab32371) were purchased from Abcam (Cambridge, MA).