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Biotinylated phycoerythrin

Manufactured by Thermo Fisher Scientific

Biotinylated phycoerythrin is a fluorescent labeling reagent. It is a conjugate of the fluorescent protein phycoerythrin and the small molecule biotin. Biotinylated phycoerythrin can be used to label proteins, cells, and other biomolecules.

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2 protocols using biotinylated phycoerythrin

1

Lipid Reconstitution and Characterization

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PI3P was from Echelon (Salt Lake City, Utah), ergosterol from Sigma (St. Louis, MO), fluorescent lipids from Thermo Fisher (Waltham, MA), and other lipids were from Avanti (Alabaster, AL). Biobeads SM2 were from BioRad, Cy5-Streptavidin from SeraCare (Milford, MA), biotinylated phycoerythrin from Invitrogen (Eugene, OR), and underivatized streptavidin from Thermo Fisher. Spectrapor six dialysis tubing (7.5 mm diameter, 25 kDa cutoff) was from Spectrum Labs (Las Vegas, NV). Octyl-b-D-glucopyranoside was purchased from Anatrace (Maumee, OH).
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2

Reconstitution of Membrane Proteins

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PI3P was from Echelon (Salt Lake City, Utah), ergosterol from Sigma (St. Louis, MO), fluorescent lipids from Thermo Fisher (Waltham, MA), and other lipids were from Avanti (Alabaster, AL). Biobeads SM2 were from BioRad, Cy5-Streptavidin from SeraCare (Milford, MA), biotinylated phycoerythrin from Invitrogen (Eugene, OR) and underivatized streptavidin from Thermo Fisher. Spectrapor 6 dialysis tubing (7.5mm dia, 25 kDa cutoff) was from Spectrum Labs (Las Vegas, NV). Octyl--D-glucopyranoside was purchased from Anatrace (Maumee, OH).
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