Anti cd14 m5e2

Manufactured by BD

Sourced in France, United States

Anti-CD14 (M5E2) is a monoclonal antibody that binds to the CD14 antigen. CD14 is a protein expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. This antibody can be used to identify and study CD14-positive cells in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd3 ucht1, by BD (2 mentions) Anti cd8 sk1, by BD (2 mentions) Lsrfortessa, by BD (2 mentions) Lsrii cytometer, by BD (2 mentions) Anti cd27 o323, by BioLegend (1 mentions) Anti cd3 sp34 2, by BD (1 mentions) Anti cd4 l200, by BD (1 mentions) Anti hla dr g46 6, by BD (1 mentions) Anti cd19 hib19, by BD (1 mentions) Anti cd123 7g3, by BD (1 mentions) Anti cd38 hit2, by BD (1 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Gentlemacstm dissociator, by Miltenyi Biotec (1 mentions) Annexin 5, by Thermo Fisher Scientific (1 mentions) Anti cd3 clone sp34 2, by BD (1 mentions) Hla dr clone g46 6, by BD (1 mentions) Collagenase d, by Roche (1 mentions) Live dead aqua marker, by Thermo Fisher Scientific (1 mentions) Trucount tube, by BD (1 mentions)

5 protocols using anti cd14 m5e2

Immune Cell Phenotyping under BSL 2+

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immune cell phenotyping was performed under BSL 2+ conditions by incubating 200 μL of fresh whole blood in polystyrene tubes with two different fluorochrome-labeled antibody panels for 20 min in the dark [Panel 1: anti-CD3 (SP34-2, #562877), anti-CD4 (L200, #560836), anti-CD8 (SK1, #341051); anti-CD19 (HIB19, #555415), anti-CD38 (HIT2, #555460), and anti-HLA-DR (G46-6, #555811) from BD, anti-CD20 (2H7, #47-0209-42) from eBioscience, and anti-CD27 (O323, #302838) from BioLegend. Panel 2: anti-CD3 (UCHT1, #557943), anti-CD11c (O33-782, 561355), anti-CD14 (M5E2, #565283), anti-CD19 (HIB19, #557921), anti-CD123 (7G3, #554529), and anti-HLA-DR (G46-6, #560651) from BD; anti-CD16 (CB16, #47-1068) and anti-CD56 (MEM188, #17-0569) from eBioscience, and anti-CD20 (2H7, #302332) from BioLegend]. Flow cytometry was performed on an LSRII (BD) and data was analyzed using FlowJo software version 9 (Tree Star). T cells expressing both HLA-DR and CD38 were considered activated.

Raabe V., Lai L., Xu Y., Huerta C., Wang D., Pouch S.M., Burke C.W., Piper A.E., Gardner C.L., Glass P.J, & Mulligan M.J. (2020). The Immune Response to Eastern Equine Encephalitis Virus Acquired Through Organ Transplantation. Frontiers in Microbiology, 11, 561530.

+ Open protocol

+ Expand

Characterizing Skin Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To characterize skin immune cells, 8 mm-biopsies were taken from the site of vaccination. The skin (dermis and epidermis) was separated from the cutaneous muscle (panniculus carnosus) and hypodermis fat tissues, and each tissue was mechanically dissociated (GentleMACS^TM dissociator, Miltenyi, France). Cells were then filtered through 70 µm nylon mesh after enzymatic treatment with 2 mg/ml of Collagenase D (Roche Diagnostics, France) for 30 minutes at 37 °C. The LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Invitrogen, France) was used to label dead cells. Cell surface staining was performed with the following antibodies: anti-CD45 (clone DO58-1283, BD, France), HLA-DR (clone G46-6, BD, France), anti-CD66abcd (clone TET2, Miltenyi Biotec, France), anti-CD3 (clone SP34-2, BD, France), anti-CD163 (clone GHI/61, Ozyme, France), anti-CD14 (M5E2, BD, France). Apoptotic cells were stained with AnnexinV and Propidium Iodide (Invitrogen, France) according to the manufacturer’s instructions. Data were acquired on LSR Fortessa (BD Biosciences, Le Pont de Claix, France) and analyzed with FlowJo software 9.4.11 (Tree Star, Ashland, OR).

Todorova B., Adam L., Culina S., Boisgard R., Martinon F., Cosma A., Ustav M., Kortulewski T., Le Grand R, & Chapon C. (2017). Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques. Scientific Reports, 7, 4122.

+ Open protocol

+ Expand

Tetramer-based Enrichment of Antigen-specific CD4+ T Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Untouched CD4⁺ T cells were isolated from PBMCs using magnetic microbeads (Miltenyi Biotec). Tetramer staining and enrichment was performed as described previously (Su et al., 2013 (link)). In brief, cells were incubated for 30 min with live/dead Aqua marker (Invitrogen), washed, and then labeled with either gE or IE63 tetramers at room temperature for 45 min (14 µg/ml). Surface markers AF700-labeled anti-CD3 (UCHT3; BD), FITC-labeled anti-CD4 (SK3; BD), Pacific blue–labeled anti-CD45RA (MHCD45RA28; BD), and PE-cyanine 7 (PECy7)–labeled anti-CD56 (B159; BD), anti-CD14 (M5E2; BD), and anti-CD8 (SK1; BD) were incubated at room temperature for 15 min. Before tetramer enrichment, 1/10th staining volume was removed and added to TruCount tubes (BD) to give an absolute count of the starting number of CD4⁺ naive and memory T cells. The remaining staining volume was enriched for tetramer-positive cells using anti-PE microbeads (Miltenyi Biotec) and added to a separate TruCount tube. Samples were acquired using an LSR Fortessa (BD), and the frequency of tetramer-positive cells determined by dividing the absolute counts of tetramer positive cells by the starting number of CD4⁺ naive/memory T cells.

Campion S.L., Brodie T.M., Fischer W., Korber B.T., Rossetti A., Goonetilleke N., McMichael A.J, & Sallusto F. (2014). Proteome-wide analysis of HIV-specific naive and memory CD4+ T cells in unexposed blood donors. The Journal of Experimental Medicine, 211(7), 1273-1280.

+ Open protocol

+ Expand

Phenotypic Profiling of Human moDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

A phenotypic analysis of human moDCs was performed for each donor after the differentiation of monocytes. Cells were stained using Zombie NIR viability dye, anti-CD14 (M5E2, BD Biosciences, Franklin Lakes, NJ, USA, RRID:AB_2687593), anti-CD209 (DCN46, BD Biosciences, Franklin Lakes, NJ, USA, RRID:AB_394123), and anti-HLA-DR (L243, BD Biosciences, Franklin Lakes, NJ, USA, RRID:AB_2738559). Cells were acquired using LSR II cytometers (BD Biosciences, Franklin Lakes, NJ, USA), and results were analyzed using FlowJo softwares (v10, Treestar, Woodburn, OR, USA). The activation state of moDC after their infection with rAAV was analyzed by flow cytometry using the following antibodies: anti-CD80 (L307.4, BD Biosciences, RRID:AB_10562564), anti CD86 (2331, BD Biosciences, Franklin Lakes, NJ, USA, RRID:AB_11153866).

Masri S., Carré L., Jaulin N., Vandamme C., Couzinié C., Guy-Duché A., Dupont J.B., Pereira A., Charpentier E., David L., Gernoux G., Guilbaud M, & Adjali O. (2023). Transcriptomic Analysis Reveals the Inability of Recombinant AAV8 to Activate Human Monocyte-Derived Dendritic Cells. International Journal of Molecular Sciences, 24(13), 10447.

+ Open protocol

+ Expand

Immunophenotyping of Tumor-Infiltrating Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor specimens were collected and freshly dissociated using enzymatic cocktail (0.1% DNaseI and Liberase 400u/ml, Roche). Leukocytes were enriched by Percoll density gradient (GE healthcare, US) and cells were banked in liquid nitrogen until further analysis. MFC was performed with a LSR II cytometer (BD Biosciences). Samples were stained with anti-CD45 (2D1, BD Biosciences) anti-CD3 (UCHT1, BD Biosciences), anti-CD4 (OKT4, BD Bioscience), anti-CD8 (RPA-T8, BD Biosciences), anti-PD-1 (EH12.1, BD Biosciences), anti-Foxp3 (259D/C7, BD Biosciences). Cytokine intracellular staining (ICS) for IFN-γ (B27, BD Biosciences) and IL-17 (SCPL1362, BD Biosciences) was performed following 3 hr -in vitro stimulation in the presence of stimulation cocktail (PMA+ionomycin; Ebioscience) and GolgiStop (Monensin; BD Biosciences) according to the manufacturer instructions. Data were analyzed using DIVA 6.1 Software (BD Biosciences). Myeloid cells were stained using anti-CD45, anti-CD11b (ICRF44, BD Biosciences), anti-DR (G46.6, BD Biosciences), anti-CD15 (HI98, BD Biosciences), anti-CD14 (M5E2, BD Biosciences), anti-CD33 (WM53, BD Biosciences), anti-CD11c (B-Ly6, BD Biosciences) and anti-PD-L1 (29E.2A3, Biolegend, San Diego, CA). Cell lines were cultured for 3 days in the presence or absence of IFN-γ (500IU/ml). Cells were stained with DR and B7-H1/PD-L1 mAb prior to analysis by flow cytometry.

Llosa N.J., Cruise M., Tam A., Wick E.C., Hechenbleikner E.M., Taube J.M., Blosser L., Fan H., Wang H., Luber B., Zhang M., Papadopoulos N., Kinzler K.W., Vogelstein B., Sears C.L., Anders R.A., Pardoll D.M, & Housseau F. (2014). The vigorous immune microenvironment of microsatellite instable colon cancer is balanced by multiple counter-inhibitory checkpoints. Cancer discovery, 5(1), 43-51.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!