For analyses of the SMs, strains were grown in submerged cultures as described above. After 7 days, mycelia were removed from the culture by filtration through Mirachloth (Calbiochem, Merck KGaA, Darmstadt, Germany). Small particulates were removed from the culture filtrates using 0.45 μm syringe filters (BGB®, Schloßböckelheim, Germany) which were then analyzed by high-pressure liquid chromatography with a diode array detector [HPLC-DAD; Hitachi Chromaster LC equipped with a 250 mm × 4.60 mm i.d., 5 μm, Gemini® C18 with a 4 mm × 3 mm Gemini® C18 guard column (Phenomenex, Aschaffenburg, Germany)], a with 5160 pump, 5260 autosampler, 5310 column oven, and 5430 DAD (VWR International GmbH, Darmstadt, Germany). For data analyses, the software EZChrom Elite (VWR, Darmstadt, Germany) was used.
5160 pump
Manufactured by Avantor
Sourced in Germany
The 5160 pump is a precision liquid handling device designed for laboratory applications. It features accurate and consistent flow rates across a range of fluid volumes. The pump is engineered to provide reliable and consistent performance in various laboratory workflows.
Automatically generated - may contain errors
Lab products found in correlation
5260 autosampler, by Avantor (2 mentions)
5310 column oven, by Avantor (2 mentions)
Gemini c18, by Phenomenex (1 mentions)
Ezchrom elite, by Avantor (1 mentions)
Gemini c18 guard column, by Phenomenex (1 mentions)
Openlab control panel software, by Agilent Technologies (1 mentions)
Liquid chromatograph, by Hitachi (1 mentions)
2 protocols using 5160 pump
1
Analysis of Secondary Metabolites via HPLC-DAD
2
HPLC and UPLC-ESI-QTOF-MS Analysis of Hyperforins and Hypericins
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The HPLC system consisted of a VWR
HITACHI liquid chromatograph equipped with a 5160 pump, a 5260 autosampler,
a 5430 diode array detector, an organizer (eluent stand), and temperature
controller for the column and sampler (5310 column oven) (VWR, HITACHI,
MA, JAPAN). The absorption was measured in the range of 200–800
nm, with the UV–visible detector working at 260 nm for hyperforins
and 350 and 590 nm for hypericins. The chromatographic data were recorded
and processed with Agilent Open LAB Control Panel software. For UPLC-ESI-QTOF-MS
analysis, a Waters Acquity high-performance liquid chromatograph (UPLC,
Waters) coupled with a time-of-flight Q-TOF micro-mass spectrometer
(Waters) and equipped with electrospray ionization (ESI) was used.
Mass spectra were recorded in the positive ion mode at 3 kV capillary
voltage and 230 °C desolvation temperature.
HITACHI liquid chromatograph equipped with a 5160 pump, a 5260 autosampler,
a 5430 diode array detector, an organizer (eluent stand), and temperature
controller for the column and sampler (5310 column oven) (VWR, HITACHI,
MA, JAPAN). The absorption was measured in the range of 200–800
nm, with the UV–visible detector working at 260 nm for hyperforins
and 350 and 590 nm for hypericins. The chromatographic data were recorded
and processed with Agilent Open LAB Control Panel software. For UPLC-ESI-QTOF-MS
analysis, a Waters Acquity high-performance liquid chromatograph (UPLC,
Waters) coupled with a time-of-flight Q-TOF micro-mass spectrometer
(Waters) and equipped with electrospray ionization (ESI) was used.
Mass spectra were recorded in the positive ion mode at 3 kV capillary
voltage and 230 °C desolvation temperature.
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