All materials for synthesis were commercially available without further purification. All solvents used were of analytical reagent grade and all aqueous solutions were prepared using pure water. The caffeic acid tablets were purchased from Dezhou Deyao Pharmaceutical Co., Ltd. The 1H NMR spectra and 13C NMR spectra were recorded on a Bruker AM-600 spectrometer (Billerica, MA), and chemical shift (δ) were given in parts per million relative to tetramethylsilane (TMS). High-resolution mass spectrometry (HRMS) spectra were recorded on an Agilent 1290LC-6540 Accurate-Mass Q-TOF by using electrospray ionization (ESI). Chromatographic datas were recorded on a Waters high-performance liquid chromatograph (Waters Corporation, USA), and the separated compounds were collected using a SHIMADZU LC-20AR preparative liquid chromatograph (Shimadzu, Japan). Ultraviolet absorption datas were collected on a HITACHI U-2910 UV-Visible spectrophotometer (Hitachi, Japan). Fluorescence datas were collected on an RF5301PC Fluorescence Spectrophotometer (Shimadzu, Japan).
U 2910 uv visible spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The Hitachi U-2910 UV-Visible spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is used to analyze and quantify the concentration of chemical species in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Rf 5301pc fluorescence spectrophotometer, by Shimadzu (1 mentions)
1290lc 6540 accurate mass q tof, by Agilent Technologies (1 mentions)
Waters high performance liquid chromatograph, by Waters Corporation (1 mentions)
Am 600 spectrometer, by Bruker (1 mentions)
F 4600 spectrophotometer, by Hitachi (1 mentions)
Victor nivo, by PerkinElmer (1 mentions)
Bx53 microscope, by Olympus (1 mentions)
3 protocols using u 2910 uv visible spectrophotometer
1
Caffeic Acid Characterization and Purification
2
Fluorescence and UV Absorption Analysis
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The F-7000 fluorescence spectrometer was used to measure the fluorescence intensity of N-LG, U-LG, S-LG–LUT and U-LG–LUT [19 (link)]. The measured parameters were: excitation wavelength at 280 nm, emission wavelength range 300–450 nm, voltage 400 V, excitation and emission slit width 5 nm. Hitachi U-2910 UV-visible spectrophotometer was used to analyze the UV absorption spectrum of N-LG, U-LG, S-LG–LUT and U-LG–LUT. The sample was diluted to 1 mg/mL with PBS (pH = 7.4, 10 mmol/L), and the scanning range was 240–400 nm.
3
Multimodal Imaging and Biophysical Characterization
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An Olympus CKX53
instrument and an Olympus BX53 microscope were used for performing
bright field, DIC, and fluorescence imaging. A Hitachi U-2910 UV–visible
spectrophotometer was used for measuring UV–visible absorption
spectra, using a quartz cell of 1 cm path length. A Hitachi F-4600
spectrophotometer was used for measuring fluorescence spectra. Size
distribution and surface charge analysis via dynamic light scattering
(DLS) were quantified using a Nano ZS (Malvern) instrument. Hemolytic
analysis was performed using a multiplate reader (PerkinElmer Victor
Nivo).
instrument and an Olympus BX53 microscope were used for performing
bright field, DIC, and fluorescence imaging. A Hitachi U-2910 UV–visible
spectrophotometer was used for measuring UV–visible absorption
spectra, using a quartz cell of 1 cm path length. A Hitachi F-4600
spectrophotometer was used for measuring fluorescence spectra. Size
distribution and surface charge analysis via dynamic light scattering
(DLS) were quantified using a Nano ZS (Malvern) instrument. Hemolytic
analysis was performed using a multiplate reader (PerkinElmer Victor
Nivo).
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