Tumor-bearing mice were randomly divided into three groups (n=6): group A, which received full-dose cisplatin (4 mg/kg IP; Amresco, Solon, OH, USA) on day 5; group B, which received EGCG (1.5 mg/mouse/day IP) for 5 days and half-dose cisplatin (2 mg/kg IP) on day 5; and group C, which received EGCG (1.5 mg/mouse/day IP) for 5 days and full-dose cisplatin (4 mg/kg IP) on day 5. Tumors were then removed after killing the mice. Tissue samples were prepared using an acid-digestion method (dispersion in dilute hydrogen chloride), and cisplatin concentration in tumor tissue was detected using graphite-furnace atomic absorption spectrophotometry (PerkinElmer).27
Cisplatin
Manufactured by Avantor
Sourced in United States
Cisplatin is a widely used platinum-based chemotherapy drug. It is a coordination complex that functions as an antineoplastic agent.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by Avantor (2 mentions)
Ethidium bromide, by Avantor (2 mentions)
Netropsin, by Avantor (2 mentions)
Hoechst 33342, by Avantor (2 mentions)
Streptavidin biosensors and kinetics buffer, by Molecular Devices (2 mentions)
Actinomycin d, by Adooq (2 mentions)
Doxorubicin, by Thermo Fisher Scientific (2 mentions)
Enzalutamide, by MedChemExpress (1 mentions)
Docetaxel, by LC Laboratories (1 mentions)
Epigallocatechin gallate (egcg), by Avantor (1 mentions)
4 protocols using cisplatin
1
Cisplatin and EGCG Combination Therapy
2
Compound Acquisition for Cancer Research
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ASC-J9® (IUPAC Name: (1E,4Z,6E)-1,7-bis(3,4-dimethoxyphenyl)-5-hydroxyhepta-1,4,6-trien-3-one) was purchased from AndroScience Corp. (San Diego, CA). Enzalutamide (IUPAC Name: 4-(3-(4-cyano-3-(trifluoromethyl)phenyl)-5,5-dimethyl-4-oxo-2-thioxoi-midazolidin-1-yl)-2-fluoro-N-methylbenzamide) was purchased from MedChem Express (Monmouth Junction, NJ, USA). Cisplatin (IUPAC Name (SP-4-2)-diamminedichloroplatinum (II)) was purchased from VWR (Radnor, PA, USA). Docetaxel (IUPAC Name: 1,7β,10β-trihydroxy-9-oxo-5β,20-epoxytax-11-ene-2α,4,13α-triyl 4-acetate 2-benzoate 13-{(2R,3S)-3-[(tert-butoxycarbonyl)amino]-2-hydroxy-3-phenylpropanoate}) was purchased from LC Laboratories (Woburn, MA, USA).
3
Characterization of DNA-binding compounds
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Actinomycin D (1) was purchased from AdooQ Bioscience (Irvine, CA). Doxorubicin (2) was purchased from ThermoFisher Scientific (Waltham, MA). Ethidium bromide (3), propidium iodide (4), Hoechst 33342 (5), netropsin (6), and cisplatin (7) were purchased from VWR (Radnor, PA).
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
4
Characterization of DNA-binding compounds
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Actinomycin D (1) was purchased from AdooQ Bioscience (Irvine, CA). Doxorubicin (2) was purchased from ThermoFisher Scientific (Waltham, MA). Ethidium bromide (3), propidium iodide (4), Hoechst 33342 (5), netropsin (6), and cisplatin (7) were purchased from VWR (Radnor, PA).
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
All compounds used were tested for purity prior to use by LCMS. Streptavidin biosensors and Kinetics Buffer (1x PBS, pH 7.4, 0.02% Tween-20, 0.1% albumin, and 0.05% sodium azide) were purchased directly from Molecular Devices (San Jose, CA). Solvents and culture media components were all purchased from VWR unless otherwise noted.
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