A549 cells (antigen donor cells) were mock-infected or infected with IAV virus or IAV-OVA virus (MOI = 10) for 10 h prior to γ-irradiation (50 Gy) to inhibit the proliferation of viruses and induce apoptosis of the cells. After washing, the irradiated cells were incubated with Mutu cells (GFP+) at a ratio of 1:1 or 1:2 in the presence of 500 nM CpG oligodeoxynucleotides (InvivoGen) at 37 °C and harvested at the designated time. For preliminary analysis of cross-presentation, the Mutu cells were then co-cultured with VPD450-labeled OT-1 CD8+ T cells at ratio of 1:1 at 37 °C for 24 h. OT-1 T-cell proliferation was assessed based on the VPD450 dye dilution in CD8+ T cells. Alternatively, the Mutu cells were stained with monoclonal 25-D1.16 antibody, specific for the H-2Kb bound peptide OVA257–264 (SIINFEKL), followed by a PE-conjugated anti-mouse IgG antibody (BD Biosciences), and analyzed by flow cytometry.
Pe conjugated anti mouse igg antibody
Manufactured by BD
The PE-conjugated anti-mouse IgG antibody is a laboratory reagent used for the detection and quantification of mouse immunoglobulin G (IgG) in various immunoassays and research applications. The antibody is conjugated with the fluorescent dye Phycoerythrin (PE), which allows for the visualization and tracking of target mouse IgG molecules.
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Lab products found in correlation
Cpg oligodeoxynucleotide, by InvivoGen (1 mentions)
Cd34 pe, by BD (1 mentions)
Cd31 pe, by BD (1 mentions)
Cd49d pe, by BD (1 mentions)
Cd57 fitc, by BD (1 mentions)
Cd13 pe, by BD (1 mentions)
Cd44 fluorescein isothiocyanate fitc, by BD (1 mentions)
Cd133 1 pe, by Miltenyi Biotec (1 mentions)
Facscalibur, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
Cd73 pe, by BD (1 mentions)
2 protocols using pe conjugated anti mouse igg antibody
1
Antigen cross-presentation in A549 cells
2
Multiparametric Characterization of HUCB and Bone Marrow Cells
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Antibodies against the human antigens CD13-PE, CD31-PE, CD34-PE, CD44-fluorescein isothiocyanate (FITC), CD49d-PE, CD57-FITC, CD73-PE, CD90 (Thy-1)-pure, and CD105 (Endoglin)-pure were purchased from BD Biosciences (San Jose, CA), CD45-FITC was from Immunotech (Marseille, France), and CD133/1-PE, and CD271(p75NTR)-FITC, -PE, -APC were obtained from Miltenyi Biotec. For each antibody expression assessment, a total of 1 × 105 MNCs from HUCB and human bone marrow were resuspended in 100 μl PBS containing 0.5% BSA and 200 mM EDTA, incubated with rabbit serum for blocking nonspecific binding. The cells were then incubated with primary antibodies for 30 min on ice. Binding of unconjugated anti-CD90 and CD105 was detected by secondary staining with PE-conjugated anti-mouse IgG antibody (BD Biosciences). FITC- and PE-conjugated mouse IgGs were used as the control isotype at the same concentration as specific primary antibodies. The fluorescence intensity of the cells was evaluated by flow cytometry (FACSCalibur, BD Biosciences), and data was analyzed with CellQuest software (BD Biosciences).
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