Anti gp91phox antibody

Xanthine, Xanthine oxidase, SQ 22,536, L798106, poly-D-lysine, superoxide dismutase (SOD), mazindol, 3,3′-diaminobenzidine and urea-hydrogen peroxide tablets were purchased from Sigma-Aldrich (St. Louis, MO, USA). Water-soluble tetrazolium salt-1 (WST-1) was purchased from Dojindo (Rockville, MD, USA). Lipopolysaccharide (LPS; E. coli strain O111: B4) was purchased from Calbiochem (San Diego, CA, USA). Cell culture ingredients were obtained from Life Technologies (Grand Island, NY, USA). Prostaglandin E₂ (PGE₂), 17-phenyl trinor prostaglandin E₂, butaprost, CAY-10598, SC-19220, AH-6809 and AH-23848 were purchased from Cayman Chemical (Ann Arbor, MI, USA). Anti-tyrosine hydroxylase (TH) was purchased from Chemicon (Billerica, MA, USA) and antibody diluent was purchased from DAKO (Carpinteria, CA, USA). TNF-α ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA). Direct cAMP ELISA kit was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Anti-p47^phox antibody was purchased from Millipore (Temecula, CA, USA). Anti-gp91^phox antibody was purchased from BD Biosciences (San Jose, CA, USA). Anti-GAPDH antibody was purchased from Abcam (Cambridge, MA, USA). Alexa Fluor 488 goat anti-rabbit IgG was purchased from Invitrogen (Carlsbad, CA, USA). Goat anti-rabbit biotinylated secondary antibody was purchased from Vector Laboratory (Burlingame, CA, USA).