Slc27a3

After treatment, lung tissues were collected. Paraffin embeded lung tissues were cut into slices for histological staining. HE staining was performed according to the manufacturer’s instructions (Solarbio). The mean alveolar septal thickness (MAST), mean linear intercept (MLI), and destructive index (DI) were calculated. Immunohistochemistry staining was conducted using primary antibodies for SLC27A3 (Proteintech), STAU1 (Affinity), CD57 (Affinity), and CD8a (Affinity). Immunofluorescence staining was performed using primary antibodies for CD19 (Affinity) and CD27 (Santa), and secondary antibodies including Cy3-labeled goat anti-rabbit IgG (Invitrogen) and FITC-labeled goat anti-mouse IgG (Abcam).

Total protein was extracted from lung tissues and BEAS-2B cells by protein extraction kit (Wanleibio). The concentration of protein was detected by BCA kit (Wanleibio). The protein was separated by SDS-PAGE and transferred to PVDF membrane (Millipore). The membrane was blocked by non-fat milk and then incubated with primary antibodies for SLC27A3 (Proteintech), STAU1 (Affinity), ACPL2 (Bioss), RABL4 (Biorbyt), and β-actin (Wanleibio), respectively. After 12-h of incubation at 4°C, the membrane was washed, incubated by the secondary antibody, and visualized by CEL (Wanleibio). β-actin serves as an internal control.