All cell culture reagents were from Invitrogen (Paisley, UK). All chemicals were purchased from Sigma‐Aldrich (St. Louis, MO) unless indicated otherwise. Neuraminidase from Clostridium perfringens was from Sigma‐Aldrich. 3‐FAx‐peracetyl‐Neu5Ac was from Merck Millipore (Burlington, MA). Carboxylated Nile red 5 μm beads were from Spherotech (Chicago, IL). CD11b‐targeting, nontargeting siRNA, Lipofectamine 3000 reagent and mouse IgG2a isotype control were purchased from Thermo Scientific (Waltham, MA). Mouse monoclonal anti‐CD11b (clone OX‐42) antibody was from Serotec AbD (Hercules, CA). Recombinant human Tau protein (isoform 2N4R) was a kind gift from Dr Vilmante Borutaite. FITC‐labeled human fibrinogen was from Molecular Innovations (Novi, MI).
3fax peracetyl neu5ac
Manufactured by Merck Group
3Fax-Peracetyl Neu5Ac is a laboratory reagent used for the synthesis and modification of sialic acid derivatives. It serves as a precursor compound in the production of various sialic acid-related compounds for research and analytical purposes.
Automatically generated - may contain errors
Lab products found in correlation
2f peracetyl fucose, by Merck Group (2 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (2 mentions)
Fitc labeled human fibrinogen, by Innovative Research (1 mentions)
Neuraminidase from clostridium perfringens, by Merck Group (1 mentions)
Cell fixation and permeabilization kit, by Thermo Fisher Scientific (1 mentions)
Carboxyfluorescein diacetate succinimidyl ester cfse, by Thermo Fisher Scientific (1 mentions)
Maackia amurensis agglutinin, by Vector Laboratories (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Golgistop, by BD (1 mentions)
Neuraminidase, by Roche (1 mentions)
Quantifast sybr green, by Qiagen (1 mentions)
Qscript cdna supermix, by Avantor (1 mentions)
Recombinant human il 1β, by Thermo Fisher Scientific (1 mentions)
Teflon tape, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Alamarblue assay, by Thermo Fisher Scientific (1 mentions)
Rnase inhibitor, by Meridian Bioscience (1 mentions)
Primocin, by InvivoGen (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
4 protocols using 3fax peracetyl neu5ac
1
Analyzing Microglial CD11b Regulation
2
Fucosylation and Sialylation Modulation in Cells
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ℒ-fucose (Sigma-Aldrich, F2252-5G) was used to reconstitute fucosylation in Slc35c1-deficient cells. Inhibition of fucosylation in HDF, HL-60, MDCK and VERO cells was achieved using 2F-Peracetyl-Fucose (Merck Chemicals and Life Science, 344827)22 (link); murine organoids were treated with 2-deoxy-2-fluoro-ℒ-Fucose (Carbosynth, MD06089)23 (link). Sialylation was inhibited using 3Fax-Peracetyl Neu5Ac (Merck Chemicals and Life Science, US1566224)22 (link).
3
Siglec-7 and Siglec-9 Binding Assay
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Siglec-7 and Siglec-9 chimeras were from R&D Systems (1138-SL-050 and 1139-SL-050), anti-human immunoglobulin G Fc fragment was from Jackson ImmunoResearch (009-000-008). Maackia amurensis agglutinin was from Vector Laboratories (B-1265), 3Fax-Peracetyl Neu5Ac was from Merck (566224-10mg), daratumumab (Dara) (Janssen) was supplied by University Hospital Galway, and neuraminidase (Vibrio cholerae) was supplied by Roche (11 080 725 001). Carboxyfluorescein diacetate succinimidyl ester (CFSE) and cell fixation and permeabilization kit were from Thermo Fisher Scientific (C34554 and 00-5523-00). Tag-it dye was from Biolegend (425101). GolgiStop was from BD Biosciences (554724). Propidium iodide (PI) was from Sigma. (Antibody details are located in Supplementary Materials.)
4
Cartilage Extracellular Matrix and Inflammatory Biomarkers
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3Fax-Peracetyl Neu5Ac and 2F-Peracetyl-Fucose were purchased from Merck. 96-well glass coverslip microplates were purchased from Ibidi. All lectins were purchased from Vector Labs. Human recombinant IL-1β, IL-6 and TNF-α were purchased from Peprotech. RNeasy Mini Kit and Quantifast SYBR Green were purchased from Qiagen. qScript cDNA SuperMix™ was purchased from VWR. RNase inhibitor was purchased from Bioline. PCR primers were purchased from Eurofins. Human MMP-13 ELISA kit was purchased from Abcam. Teflon™ tape, Live/Dead ® staining kit, alamarBlue ® assay, DAPI, TaqMan ® VIC ® -labelled GAPDH primer and TaqMan ® Universal PCR Master Mix, no AmpErase ® UNG were purchased from Fisher Scientific. Primocin was purchased from InvivoGen. TaqMan FAM™ labelled COL1/2 primers were purchased from Thermo Fisher Scientific. All other materials and reagents were purchased from Sigma-Aldrich unless otherwise stated.
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