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Irdye 680cw

Manufactured by Thermo Fisher Scientific
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Sourced in Germany

The IRDye 680CW is a near-infrared fluorescent dye designed for use in a variety of in vitro and in vivo biological applications. It has an excitation maximum of 680 nm and an emission maximum of 700 nm, making it suitable for detection using near-infrared imaging systems.

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Lab products found in correlation

Ma5 11869, by Thermo Fisher Scientific (2 mentions) Bis tris gel, by GenScript (2 mentions) Protran ba85 nitrocellulose membrane, by Cytiva (2 mentions) Pa5 69577, by Thermo Fisher Scientific (2 mentions) Irdye 800cw, by Thermo Fisher Scientific (2 mentions)

2 protocols using irdye 680cw

1

Immunoblotting Analysis of POLQ Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
A portion of 293T cells from the MMEJ reporter assays performed after overexpression of POLQWT or POLQ-DY2230AA polymerase mutant was used for western blotting analysis. Cells were resuspended in IP lysis buffer (Cat. No: 87787, Thermo scientific, USA) and laemmli buffer was used to make whole-cell protein extracts. Equal amounts (20 μg) whole-cell protein lysates were separated on 4–20% bis tris gels (GenScript) by electrophoresis then transferred onto Protran BA85 nitrocellulose membrane (Whatman, Germany) and immunoblotted with antibodies against Actin (MA-5-11869,1:20000, Invitrogen) or POLQ (PA5-69577,1:500, Invitrogen) overnight followed by secondary antibodies IRDye 800CW (926-32210, 1:10000) or IRDye 680CW (926-68073, 1:10000). Blots were scanned using ODYSSEY software.
Chandramouly G., Liao S., Rusanov T., Borisonnik N., Calbert M.L., Kent T., Sullivan-Reed K., Vekariya U., Kashkina E., Skorski T., Yan H, & Pomerantz R.T. (2021). Polθ promotes the repair of 5′-DNA-protein crosslinks by microhomology-mediated end-joining. Cell reports, 34(10), 108820.
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2

Immunoblotting Analysis of POLQ Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
A portion of 293T cells from the MMEJ reporter assays performed after overexpression of POLQWT or POLQ-DY2230AA polymerase mutant was used for western blotting analysis. Cells were resuspended in IP lysis buffer (Cat. No: 87787, Thermo scientific, USA) and laemmli buffer was used to make whole-cell protein extracts. Equal amounts (20 μg) whole-cell protein lysates were separated on 4–20% bis tris gels (GenScript) by electrophoresis then transferred onto Protran BA85 nitrocellulose membrane (Whatman, Germany) and immunoblotted with antibodies against Actin (MA-5-11869,1:20000, Invitrogen) or POLQ (PA5-69577,1:500, Invitrogen) overnight followed by secondary antibodies IRDye 800CW (926-32210, 1:10000) or IRDye 680CW (926-68073, 1:10000). Blots were scanned using ODYSSEY software.
Chandramouly G., Liao S., Rusanov T., Borisonnik N., Calbert M.L., Kent T., Sullivan-Reed K., Vekariya U., Kashkina E., Skorski T., Yan H, & Pomerantz R.T. (2021). Polθ promotes the repair of 5′-DNA-protein crosslinks by microhomology-mediated end-joining. Cell reports, 34(10), 108820.
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