The IgG anti-mouse CD47 and IgG anti-mouse PD-L1 blockade monoclonal antibodies (Clone: MIAP301 and 10F.9G2 respectively) were purchased from Bioxcell (NH, USA). PerCP Rat anti-mouse CD4 antibody (RM4-5 clone), PE-labeled Rat anti-mouse CD8a antibody(53-6.7 clone), PE-labeled Rat anti-mouse CD25 antibody (PC61 clone), Alexa-flour® 488 labeled Rat anti-mouse Foxp3 antibody (MF-14 clone), Alexa Fluor® 488 anti-mouse CD47(MIAP301), PE anti-mouse CD274 (B7-H1, PD-L1) antibody (10F.9G2 clone), PerCPanti-mouse/human CD11b antibody (M1/70 clone), Alexa Fluor® 488 anti-mouse CD80 antibody (16-10-A1 clone), PE anti-mouse CD206 (MMR) antibody (C068C2 clone), APC labeled anti-mouse CD45 antibody (30F11 clone), NIR Zombie die (for live/Dead discrimination), as well as appropriate isotype control antibodies and a True-Nuclear™ transcription factor buffer set were purchased from Biolegend (CA, San Diego). Collagenase type I was purchased from Gibco (NY, USA). DNase type I was purchased from Roche (USA). The remaining chemical solvents and reagents were chemical grade.
Pe labeled rat anti mouse cd8a antibody
Manufactured by BioLegend
The PE-labeled Rat anti-mouse CD8a antibody is a laboratory reagent used for the detection and analysis of CD8a-positive cells in mouse samples. It is a fluorescently-labeled monoclonal antibody that specifically binds to the mouse CD8a surface antigen.
Automatically generated - may contain errors
Lab products found in correlation
True nuclear transcription factor buffer set, by BioLegend (2 mentions)
Dnase type 1, by Roche (2 mentions)
Apc labeled anti mouse cd45 antibody, by BioLegend (2 mentions)
Collagenase type 1, by Thermo Fisher Scientific (2 mentions)
Pe anti mouse cd206 mmr antibody, by BioLegend (1 mentions)
Mpeg2000 dspe, by Lipoid (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using pe labeled rat anti mouse cd8a antibody
1
Immune Checkpoint Blockade Antibodies
2
Immune Checkpoint Blockade Antibody Protocol
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Hydrogenated soya phosphatidylcholine (HSPC) and Methoxypolyetheleneglycol (Mw 2000) distearylphosphatidylethanolamine (mPEG2000-DSPE) were purchased from Lipoid (Ludwigshafen, Germany). Cholesterol was purchased from Sigma–Aldrich (St. Louis, MO). Commercially available Caelyx® was purchased from the Behestan Darou Company (Tehran, Iran). The IgG anti-mouse CTLA-4 blockade monoclonal antibody (Clone: 9H10) was purchased from Bioxcell (NH, USA). Pe-Cy5-labeled Rat anti-mouse CD4 antibody, PE-labeled Rat anti-mouse CD8a antibody, PE-labeled Rat anti-mouse CD25 antibody, Alexa-flour labeled Rat anti-mouse Foxp3 antibody, APC labeled anti-mouse CD 45 antibody, NIR Zombie die (for live/Dead discrimination), as well as appropriate isotype control antibodies and a True-Nuclear™ transcription factor buffer set were purchased from Biolegend (CA, San Diego). Collagenase type I, RPMI 1560 medium, and Fetal Bovine Serum (FBS) was purchased from Gibco (NY, USA). Dnase type I was purchased from Roche (USA). The remaining chemical solvents and reagents were chemical grade.
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