The LAMP assay was conducted with the primers F3, B3, FIP, BIP, FL, and BL, as previously described [15 (link), 16 (link)]. The LAMP reaction was performed with a Loopamp DNA amplification kit® (Eiken Chemical, Tokyo, Japan) using reaction mixtures composed of 40 pmol each of primers FIP and BIP, 5 pmol each of primers F3 and B3, 20 pmol each of primers FL and BL; 12.5 μL of 2 × reaction mixture; 1 μL of Bst deoxyribonucleic acid (DNA) polymerase; 2 μL of DNA sample; and distilled water up to a final volume of 25 μL. The mixtures were incubated at 61 °C for 60 min (Realoop-30; Eiken Chemical) and then heated at 80 °C for 2 min to terminate the reaction. The positive control solution for Pneumocystis jirovecii plasmid DNA (6 × 106 copies per tube) [15 (link)] and negative control solutions of Tris–EDTA buffer were also prepared with each assay. LAMP products were detected by real-time turbidity detection using a Loopamp EXIA® (Eiken Chemical, Tokyo, Japan).
Realoop 30
Manufactured by Eiken Chemical
Sourced in Japan
The Realoop-30 is a laboratory equipment designed for performing loop-mediated isothermal amplification (LAMP) reactions. It provides a controlled and consistent thermal environment for the amplification of nucleic acid samples. The Realoop-30 is capable of maintaining a specific temperature range with high accuracy, ensuring reliable and reproducible results during the LAMP process.
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Lab products found in correlation
2 protocols using realoop 30
1
LAMP Assay for Pneumocystis jirovecii
2
Molecular Detection of Vibrio Pathogenicity Genes
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tdh gene: Loop-mediated isothermal amplification assay was applied to detect tdh gene using a Loopamp DNA amplification kit (Eiken Chemical Co., Ltd.,
Tokyo, Japan) as described by Yamazaki et al. [25 (link)]. The incubation was carried out in a Loop realtime tubidimeter (Realoop-30, Eiken
Chemical) at 65°C for 60 min, following by 80°C for 2 min. A reaction was considered positive as the turbidity reached 0.1 within 60 min.
trh gene: PCR assay for trh gene examination was performed using the protocol described previously [2 (link)]. PCR amplified
products were checked in 1.5% agarose gels by electrophoresis. After that, the gel was stained with ethidium bromide (0.5 mg/ml) and photographed under a UV
transilluminator.
Tokyo, Japan) as described by Yamazaki et al. [25 (link)]. The incubation was carried out in a Loop realtime tubidimeter (Realoop-30, Eiken
Chemical) at 65°C for 60 min, following by 80°C for 2 min. A reaction was considered positive as the turbidity reached 0.1 within 60 min.
trh gene: PCR assay for trh gene examination was performed using the protocol described previously [2 (link)]. PCR amplified
products were checked in 1.5% agarose gels by electrophoresis. After that, the gel was stained with ethidium bromide (0.5 mg/ml) and photographed under a UV
transilluminator.
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