Streptavidin coated beads

Manufactured by Merck Group

Streptavidin-coated beads are a type of solid support matrix used in various biomedical applications. Streptavidin, a protein derived from the bacterium Streptomyces, is covalently attached to the surface of these beads, enabling the capture and immobilization of biotinylated molecules. The high-affinity interaction between streptavidin and biotin is the core function of these beads, which can be utilized in a range of research and diagnostic procedures.

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Lab products found in correlation

Nigericin, by Merck Group (2 mentions) Anti mouse caspase 1 p20 ag 20b 0042, by Adipogen (2 mentions) Pam3csk4, by Thermo Fisher Scientific (2 mentions) Protein g agarose, by Merck Group (2 mentions) Ultrapure lps, by Thermo Fisher Scientific (2 mentions) Anti nek7 sc 50756, by Santa Cruz Biotechnology (2 mentions) Anti asc sc 22514 r, by Santa Cruz Biotechnology (2 mentions) Anti β actin p30002, by Abmart (2 mentions) Anti human cleaved il 1β a5208206, by Sangon (2 mentions) Anti flag f2555, by Merck Group (2 mentions) Anti mouse il 1β p17 af 401 na, by R&D Systems (2 mentions) Anti nlrp3 ag 20b 0014, by Adipogen (2 mentions) Anti human caspase 1, by Cell Signaling Technology (2 mentions) Mitosox, by Thermo Fisher Scientific (2 mentions) Mitotracker, by Thermo Fisher Scientific (2 mentions) Adenosine triphosphate (atp), by Merck Group (2 mentions) Recombinant human insulin, by Novo Nordisk (1 mentions) Poly da dt, by Merck Group (1 mentions) Mcc950, by Selleck Chemicals (1 mentions) Dl sulforaphane, by Merck Group (1 mentions)

Spelling variants of this product

Streptavidin beads (58 citations) Streptavidin-coated magnetic beads (37 citations) M-280 streptavidin-coated magnetic beads (8 citations)

2 protocols using streptavidin coated beads

NLRP3 Inflammasome Activation Pathway

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MSU, nigericin, ATP, PMA, BHB, DL-sulforaphane, poly(dA:dT), and glucose were purchased from Sigma-Aldrich. MCC950 was acquired from Selleck. BAY 11-7082, isoliquiritigenin, parthenolide, flufenamic acid, and mefenamic acid were acquired from Topscience. Human recombinant insulin was bought from Novo Nordisk. The One Touch Ultra Blood glucose Test System kit was bought from Roche. Ultrapure LPS, Pam3CSK4, MitoTracker, and MitoSOX were obtained from Invitrogen. Protein G agarose and streptavidin-coated beads were respectively supplied by Millipore and Pierce Biochemicals. ATP-coupled beads were from BioWorld Company. Anti-mouse caspase-1 (p20; AG-20B-0042) and anti-NLRP3 (AG-20B-0014) were from Adipogen. Anti-ASC (sc-22514-R) and anti-NEK7 (SC-50756) were from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17; AF-401-NA) was from R&D Systems. Anti-Flag (F2555) and anti-Mcherry (V4888) were from Sigma-Aldrich. Recombinant human NLRP3 was from Novus Biologicals. A standard HFD (D12492, 60% kcal fat) was from Research Diet Company.

Jiang H., He H., Chen Y., Huang W., Cheng J., Ye J., Wang A., Tao J., Wang C., Liu Q., Jin T., Jiang W., Deng X, & Zhou R. (2017). Identification of a selective and direct NLRP3 inhibitor to treat inflammatory disorders. The Journal of Experimental Medicine, 214(11), 3219-3238.

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NLRP3 Inflammasome Activation and IL-1β Regulation

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ATP, MSU, poly A:T, nigericin, and glucose were from Sigma. The human recombinant insulin was from Nova Nordisk. One Touch^® Ultra^® Blood glucose Test System was from Roche. Oridonin (s2335) was bought from Selleck. MitoSOX, ultrapure LPS, Pam3CSK4 and MitoTracker were from Invitrogen. Protein G agarose and streptavidin-coated beads were, respectively, supplied by Millipore and Pierce Biochemicals. Anti-Flag (F2555) and anti-VSV (V4888) were bought from Sigma. Anti-NLRP3 (AG-20B-0014) and anti-mouse caspase-1 (p20) (AG-20B-0042) antibodies were from Adipogen. Anti-NEK7 (SC-50756) and anti-ASC (sc-22514-R) antibodies were obtained from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D Systems. A standard high-fat diet (D12492, 60% kcal fat) was from Research Diet Company.

He H., Jiang H., Chen Y., Ye J., Wang A., Wang C., Liu Q., Liang G., Deng X., Jiang W, & Zhou R. (2018). Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity. Nature Communications, 9, 2550.

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