Human and pig male proximal tubular cell lines (HK2, ATCC, CRL-2190; LLC-PK1, ATCC, CL-101) were obtained from the Cell Culture Facility (CCF) at Duke University. Wild-type and Nrf2 knockout male mouse embryonic fibroblasts (MEFs) were a generous gift from Drs. Wakabayashi, Yagishita, and Kensler (Wakabayashi et al., 2010 (link)). Cells were seeded on 96-well tissue culture assay plates at 2,500 cells/well with 3 biological replicates per condition, and they were cultured with standard methods in a humidified incubator at 37°C with 5% CO2. HK2 and LLC-PK1 cells were cultured in DMEM high glucose (Corning) supplemented with 10% fetal bovine serum (Corning, 35–010-CV) and 1% penicillin/streptomycin (Sigma). MEFs were cultured in IMDM (Thermo, 76,050) supplemented with 10% fetal bovine serum and 1% primocin (Invivogen, ANT-PM-1) (Wakabayashietal.,2010 (link)). Cells were tested to be mycoplasma free by Duke CCF. The cell lines have not been authenticated in our lab.
Llc pk1
Manufactured by Corning
LLC-PK1 is a cell line derived from the kidney of a normal male pig. It is commonly used as an in vitro model for the study of various kidney-related processes and functions.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Merck Group (2 mentions)
High glucose dmem, by Corning (2 mentions)
Crl 2190, by American Type Culture Collection (2 mentions)
Llc pk1, by American Type Culture Collection (2 mentions)
Cl 101, by American Type Culture Collection (2 mentions)
Ant pm 1, by InvivoGen (2 mentions)
2 protocols using llc pk1
1
Cell Lines for Nrf2 Knockout Studies
2
Cell Lines for Nrf2 Knockout Studies
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Human and pig male proximal tubular cell lines (HK2, ATCC, CRL-2190; LLC-PK1, ATCC, CL-101) were obtained from the Cell Culture Facility (CCF) at Duke University. Wild-type and Nrf2 knockout male mouse embryonic fibroblasts (MEFs) were a generous gift from Drs. Wakabayashi, Yagishita, and Kensler (Wakabayashi et al., 2010 (link)). Cells were seeded on 96-well tissue culture assay plates at 2,500 cells/well with 3 biological replicates per condition, and they were cultured with standard methods in a humidified incubator at 37°C with 5% CO2. HK2 and LLC-PK1 cells were cultured in DMEM high glucose (Corning) supplemented with 10% fetal bovine serum (Corning, 35–010-CV) and 1% penicillin/streptomycin (Sigma). MEFs were cultured in IMDM (Thermo, 76,050) supplemented with 10% fetal bovine serum and 1% primocin (Invivogen, ANT-PM-1) (Wakabayashietal.,2010 (link)). Cells were tested to be mycoplasma free by Duke CCF. The cell lines have not been authenticated in our lab.
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