Vacutainers with sodium heparin

Peripheral whole blood (≥ 5mL) from a total of 23 unique healthy human donors (7 males, 16 females, aged 22–63 years old, no X-ray exposure in the past 6 months, written consent obtained, approved by Columbia University IRB AAAS7621) was collected by venipuncture BD Vacutainers® with Sodium-Heparin (BD Biosciences 367878). Blood samples from each donor were aliquoted and mock irradiated or X-irradiated by X-RAD 320 biological irradiator (Precision X-Ray Inc., North Branford, CT) up to total doses of 1, 2, 3, 4, or 5 Gy, with the following conditions: custom house made filter: 1.5mmAl 0.25Cu 1.25Sn, 320 kVp, 12.5 mA, FSD 40, 0.95 Gy/min. Dose rate was validated with an ion chamber (Radcal® 10X6-6) before each sample irradiation.

Peripheral whole blood samples from a total of 23 unique healthy adult human donors (recruited between October 12^th 2021 and March 20^th 2023, aged 21–63 years old, 12 females, 11 males, with no X-ray exposure in the past 6 months, written consent obtained, approved by Columbia IRB AAAS7621) were collected by venipuncture in BD Vacutainers^® with Sodium-Heparin (BD Biosciences 367878). At ~1–3 hours after collection, blood samples were mock or X-irradiated with 1, 2, 3, 4, or 5 Gy at CRR inside 50 mL conical tubes placed horizontally (with the blood spread into an even layer) on the shelf of an X-RAD 320 biological irradiator (Precision X-Ray Inc., North Branford, CT) operated at 320 kVp, 12.5 mA with a custom Thoreaus filter (1.5 mm Al, 0.25 mm Cu, 1.25 mm Sn,; HVL 4 mm Cu), and a Focus-to-Surface Distance (FSD) of 40 cm. The dose rate was 0.95 Gy/min. Dosimetry was performed prior to irradiating each batch of samples using an annually calibrated 10x6-6 ion chamber (Radcal, Monrovia, CA).