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The MC57G is a cell line derived from a murine fibrosarcoma. It is a useful tool for researchers studying cellular processes and signaling pathways.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Corning (2 mentions) Dulbecco modified eagle medium (dmem), by Corning (2 mentions) Roswell park memorial institute (rpmi), by Corning (2 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Avantor (1 mentions) Nih3t3, by American Type Culture Collection (1 mentions) Jurkat cells clone e6 1, by American Type Culture Collection (1 mentions) Ccl 10, by American Type Culture Collection (1 mentions)

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MC57G cells (3 citations)

5 protocols using mc57g

1

Generation and Characterization of Murine Dendritic Cells

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Immature bone marrow-derived dendritic cells (BMDCs) were generated as previously described31 (link) with the following modifications: media changes were performed every 3 days and BMDCs were harvested on day 8. The DC2.4 cell line was maintained in RPMI (Corning Cellgro) supplemented with 10% FBS (Gibco), 1× penicillin/streptomycin(Fisher Scientific), 1× L-glutamine (Fisher Scientific), and 0.05 mM 2-mercaptoethanol (2-ME; Gibco). The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Mouse Interferon Gamma (MuIFN-γ), NR-3081. For ELISpot assays, DC2.4 were pretreated with 2 International Units (IU)/ml MuIFN-γ for 48 hours to upregulate MHCII expression. B6 fibroblast cell line has been described previously37 (link). Class II, major histocompatibility complex, transactivator (CIITA)-transduced B6 fibroblasts, MC57G (ATCC), L929 (ATCC), and I-Ab transduced L929 (L-IAb) cell lines were maintained in DMEM (Corning Cellgro) supplemented with 10% FBS and 0.05 mM 2-ME. All cell lines were periodically surveyed for mycoplasma using a commercial detection kit (Agilent technologies, catalog no. 302109)
Miller M.A., Ganesan A.P., Luckashenak N., Mendonca M, & Eisenlohr L.C. (2015). Endogenous antigen processing drives the primary CD4+ T cell response to influenza. Nature medicine, 21(10), 1216-1222.
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2

Generation and Characterization of Murine Dendritic Cells

Cited 1 time
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Immature bone marrow-derived dendritic cells (BMDCs) were generated as previously described31 (link) with the following modifications: media changes were performed every 3 days and BMDCs were harvested on day 8. The DC2.4 cell line was maintained in RPMI (Corning Cellgro) supplemented with 10% FBS (Gibco), 1× penicillin/streptomycin(Fisher Scientific), 1× L-glutamine (Fisher Scientific), and 0.05 mM 2-mercaptoethanol (2-ME; Gibco). The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Mouse Interferon Gamma (MuIFN-γ), NR-3081. For ELISpot assays, DC2.4 were pretreated with 2 International Units (IU)/ml MuIFN-γ for 48 hours to upregulate MHCII expression. B6 fibroblast cell line has been described previously37 (link). Class II, major histocompatibility complex, transactivator (CIITA)-transduced B6 fibroblasts, MC57G (ATCC), L929 (ATCC), and I-Ab transduced L929 (L-IAb) cell lines were maintained in DMEM (Corning Cellgro) supplemented with 10% FBS and 0.05 mM 2-ME. All cell lines were periodically surveyed for mycoplasma using a commercial detection kit (Agilent technologies, catalog no. 302109)
Miller M.A., Ganesan A.P., Luckashenak N., Mendonca M, & Eisenlohr L.C. (2015). Endogenous antigen processing drives the primary CD4+ T cell response to influenza. Nature medicine, 21(10), 1216-1222.
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3

Cell Culture Protocol for HEK293T, HeLa, and More

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HEK293T, HeLa cells, and Phoenix cells were obtained from the UC Berkeley Cell Culture Facility. NIH 3T3, BJAB, and MC57G cells were purchased from ATCC. Except for BJAB, all cells were cultured in DMEM (Gibco, 11995073) containing 10% FBS (VWR, 89510–186) and 1% Pen Strep (Gibco, 15140–122). BJAB cells were cultured in RPMI (Gibco, 11875) containing 10% FBS and 1% Pen Strep. Cells were cultured at 37 °C in 5% CO2.
Vargas-Zapata V., Geiger K.M., Tran D., Ma J., Mao X., Puschnik A.S, & Coscoy L. (2022). SARS-CoV-2 Envelope-mediated Golgi pH dysregulation interferes with ERAAP retention in cells. bioRxiv.
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4

Cell Culture Conditions for Immunology Research

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MC57G and Jurkat cells (clone E6-1) were obtained from ATCC. Raji cells and 293T cells were provided by J. Lieberman (Boston Children’s Hospital, Boston, MA), and N. Hacohen (Massachusetts General Hospital, Boston, MA), respectively. Raji and Jurkat cells were cultured in RPMI-1640 supplemented with 10% FBS, 2 mM l-glutamine, 50 µM β-mercaptoethanol (β-ME), 10 U/ml penicillin/streptomycin, 1 mM sodium pyruvate, and 100 mM Hepes. MC57G and 293T cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% FBS, 2 mM l-glutamine, 10 U/ml penicillin and streptomycin, and 1 mM sodium pyruvate. All cell culture reagents were obtained from Life Technologies unless otherwise noted.
Hu Y., Kim J.H., He K., Wan Q., Kim J., Flach M., Kirchhausen T., Vortkamp A, & Winau F. (2016). Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. The Journal of Experimental Medicine, 213(12), 2759-2772.
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5

LCMV Clone 13 Propagation on BHK21 Cells

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LCMV clone 13 was propagated on baby hamster kidney 21 cells (BHK21 [C13] (ATCC® CCL10). Viral titers of virus stocks were determined as described previously by using MC57G (ATCC® CRL-2295) cells48 (link).
Sandu I., Cerletti D., Claassen M, & Oxenius A. (2020). Exhausted CD8+ T cells exhibit low and strongly inhibited TCR signaling during chronic LCMV infection. Nature Communications, 11, 4454.
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