3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dex

Wild-type C57BL/6 male mice were purchased from (Orient-Bio, Seongnam, Korea). Fetal bovine serum (FBS), horse serum (HS) and Dulbecco modified Eagle's medium (DMEM) were purchased from Thermo Scientific (Waltham, MA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), DEX and all other chemicals were from Sigma-Aldrich (St. Louis, MO). Antibodies were purchased as following: Myosin heavy chain (MHC, Developmental Studies Hybridoma Bank (DSHB), Iowa, IA), MyoD (Novus biologicals, Littleton, CO), Myogenin, Myoglobin, total-OxPHOS (Abcam, Cambridge, MA), total AKT, phospho-AKT, total p38, phospho-p38, β-actin (Cell Signaling Technology, Beverly, MA), MuRF1, Atrogin-1, HSP90 (Santa Cruz Biotechnology, Santa Cruz, CA), PGC-1α (Calbiochem, San Diego, CA) and β-tubulin (Zymed, South San Francisco, CA).

Wild-type C57BL/6 male mice were purchased from (Orient-Bio, Seongnam, Korea). Fetal bovine serum (FBS), horse serum (HS), and Dulbecco modified Eagle's medium (DMEM) were purchased from Thermo Scientific (Waltham, MA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), DEX, and all other chemicals were from Sigma-Aldrich (St. Louis, MO, USA). Antibodies were purchased as follows: myosin heavy chain (MHC, Developmental Studies Hybridoma Bank (DSHB), Iowa, IA, USA) Myogenin, Myoglobin, total-OxPHOS (Abcam, Cambridge, MA, USA), total-Akt, phospho-Akt, phospho-mTOR, mTOR, phospho-S6K, S6K (Cell Signaling Technology, Beverly, MA, USA), MuRF1, Atrogin-1, HSP90 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and β-tubulin (Zymed, South San Francisco, CA, USA).