Soya lecithin and cholesterol were purchased from Acros (USA) and Merck (Germany), respectively. Celecoxib was kindly donated by Exir pharmaceutical Co., Iran. All of the solvents were of the analytical grade. Dialysis bag was purchased from the Teb Azma Co. (Tehran, Iran). Minitab 16 software was used to generate and evaluate the experimental design, and evaluate the effect of variables on responses.
Soya lecithin
Manufactured by Thermo Fisher Scientific
Sourced in United States, India
Soya lecithin is a natural emulsifier derived from soybean oil. It is a complex mixture of phospholipids and is commonly used as a food additive to improve texture, stability, and shelf life of various products.
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Lab products found in correlation
2 protocols using soya lecithin
1
Lecithin-Cholesterol Protocol for Drug Evaluation
2
Baclofen Liposomes Preparation and Characterization
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Baclofen was a provided as gift sample from Sun Pharmaceutical Industries Limited, India. Soya lecithin was purchased from Acros Organics, New Delhi, India. Cholesterol was purchased from Sigma, USA. Stearic acid purchased from Research Lab, Mumbai India. Ethanol (AR), Methanol and Chloroform purchased from Merck, India. The all other materials used in this study were of analytical grade.
The baclofen liposomes prepared as per method reported A. Kumar, et al., (2010) with slight modifications. For the preparation of liposome various formulations were prepared using different ratio of lecithin, Cholesterol and ethanol but drug and stearic acid ratio kept constant (Table 1). The lecithin, Cholesterol, stearic acid and drug were dissolving in organic solvents (ethanol) and injected in to 10 ml preheated PBS water at 55-65 o C with continuous stirring at 500 rpm using magnetic stirrer. The solvent was evaporated by heating so as to obtain drug loaded liposomes (11) .
The baclofen liposomes prepared as per method reported A. Kumar, et al., (2010) with slight modifications. For the preparation of liposome various formulations were prepared using different ratio of lecithin, Cholesterol and ethanol but drug and stearic acid ratio kept constant (Table 1). The lecithin, Cholesterol, stearic acid and drug were dissolving in organic solvents (ethanol) and injected in to 10 ml preheated PBS water at 55-65 o C with continuous stirring at 500 rpm using magnetic stirrer. The solvent was evaporated by heating so as to obtain drug loaded liposomes (11) .
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