The hydrodynamic sizes and the zeta potential of biosynthesized AgNPs in solution were analyzed using a Nanotrac Wave instrument (Microtrac). Three milliliter of sample was transferred in the clear disposable zeta cell for the measurement of zeta potential. Measurements were made by means of dynamic light scattering (DLS) in the range of 0.1–1000 µm at 25 °C, using laser wavelength of 780 nm and a scattering angle of 90°. The DLS data were analyzed by the Microtrac FLEX operating software (Montgomeryville, PA, USA).
Nanotrac wave instrument
Manufactured by Microtrac
Sourced in United States
The Nanotrac Wave is a laser diffraction particle size analyzer designed to measure the size distribution of particles in the nanometer to micrometer range. It utilizes the principle of dynamic light scattering to determine the hydrodynamic size of particles suspended in a liquid medium. The instrument provides accurate and reproducible results for a wide range of materials, including nanoparticles, emulsions, and suspensions.
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Lab products found in correlation
2 protocols using nanotrac wave instrument
1
Characterization of Biosynthesized AgNPs
2
Characterization of ZnO Phytonanoparticles
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The identification of the production of the ZnO phytonanoparticles was first evaluated by the color change of the aqueous solution of M. oleífera by Abdelmoteleb et al. [7 (link)].
For the characterization of ZnO phytonanoparticles, ultraviolet-visible spectroscopy (UV-Vis) and infrared transmission spectroscopy with Fourier transform (FT-IR) were first carried out. This was verified by performing a spectral scan of the colloidal solution in a range of 300–700 nm in a UV-Vis spectrophotometer (Beckman Coulter, USA), calibrated with a ZnSO4 solution (0.34 m) [7 (link)]. Likewise, the presence of the bands corresponding to the zinc metallic bonds in the phytonanoparticles was identified with an infrared spectroscope (NICOLET, Wisconsin, USA).
The size of the phytonanoparticles and the zeta potential were analyzed in solution using a nanotrac wave instrument (Microtrac, Montgomeryville PA, USA) according to Mendez-Trujillo et al. [8 (link)]. Measurements were made using the dynamic light scattering (DLS) technique in a range of 0.1–1000 μm at 25°C, with a laser wavelength of 780 nm and a scattering angle of 90 Åã. Data obtained from DLS was analyzed using Microtrac FLEX operating software [9 (link)–11 (link)].
For the characterization of ZnO phytonanoparticles, ultraviolet-visible spectroscopy (UV-Vis) and infrared transmission spectroscopy with Fourier transform (FT-IR) were first carried out. This was verified by performing a spectral scan of the colloidal solution in a range of 300–700 nm in a UV-Vis spectrophotometer (Beckman Coulter, USA), calibrated with a ZnSO4 solution (0.34 m) [7 (link)]. Likewise, the presence of the bands corresponding to the zinc metallic bonds in the phytonanoparticles was identified with an infrared spectroscope (NICOLET, Wisconsin, USA).
The size of the phytonanoparticles and the zeta potential were analyzed in solution using a nanotrac wave instrument (Microtrac, Montgomeryville PA, USA) according to Mendez-Trujillo et al. [8 (link)]. Measurements were made using the dynamic light scattering (DLS) technique in a range of 0.1–1000 μm at 25°C, with a laser wavelength of 780 nm and a scattering angle of 90 Åã. Data obtained from DLS was analyzed using Microtrac FLEX operating software [9 (link)–11 (link)].
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