Free fatty acids kits

Blood samples from each calf were collected every three days (d1, d4, d7, d10, d13, d16, d19, d22, d25, d28) at 1400 h from jugular venepuncture into non-heparinized vacutainers (20 mL; Becton-Dickinson, Belliver Industrial Estate, PL6 7BP, Plymouth, UK) and ethylenediaminetetraacetic acid-treated vacutainers (4 mL; Becton-Dickinson, Franklin Lakes, NJ, USA) for each experiment. Serum samples were obtained from blood after centrifugation at 2,700 × g at 4°C for 15 minutes. Serum was transferred to 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at -80°C until further analysis. The samples of blood serum were analysed for glucose (Fuji Dri-Chem Slide Glu-PⅢ, Fujufilm Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PⅢ, Fujufilm Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyser (Fuji Film, Tokyo, Japan). The non-esterified fatty acids (NEFA) was analysed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyser (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyser (Sykam S433, Sykam GmbH, Germany).

Blood samples were collected every three days (d1, 4, 7, 10, 13, 16, 19, 22, 25, and 28) at 1400 h from jugular venepuncture using non-heparinized vacutainers (20 mL; Becton-Dickinson, Belliver Industrial Estate, PL6 7BP, Plymouth, UK) and ethylenediaminetetraacetic acid-treated vacutainers (4 mL; Becton-Dickinson, Franklin Lakes, NJ, USA) 20 . Serum samples were obtained from blood after centrifugation at 2,700 × g at 4°C for 15 minutes.
Serum was transferred to a 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at -80°C until further analysis. Serum was analyzed for glucose (Fuji Dri-Chem Slide Glu-PⅢ, Fuji Film Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PⅢ, Fuji Film Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyzer (Fuji Film, Tokyo, Japan). Non-esterified fatty acids (NEFA) was analyzed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyzer (Sykam S433, Sykam GmbH, Germany).