After preparing the slides, three slides from each specimen were selected randomly. For immunohistochemical analysis, tissue were stained with anti-IL-6 antibody (1:800; Abcam, Cambridge, UK), p-NF-κB antibody (1:200; Cell Signaling Technology), and anti-Col1A1 antibody (1: 100; Santa Cruz Biotechnology). After washing three times with phosphate-buffered saline (PBS), the tissues were stained with secondary antibodies according to the manufacturer’s instructions and incubated overnight at room temperature (GBI Labs, Bothell, WA, USA). The images were analyzed using an optical microscope. Three points were randomly selected from each slide and quantified using the Image J software (National Institutes of Health, Betheshda, MD, USA).
P nf κb antibody
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
The P-NF-κB antibody is a research-use-only product that detects phosphorylated NF-κB, a key transcription factor involved in cellular signaling pathways. This antibody can be used to monitor the activation state of NF-κB in various experimental systems.
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Lab products found in correlation
2 protocols using p nf κb antibody
1
Immunohistochemical Analysis of Inflammatory Markers
2
Western Blot Analysis of Protein Signaling
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The protein was separated in SDS-PAGE system according to the instructions of the existing literature (14 (link)). The protein antibodies used were as follows: P-NF-κB antibody (diluted at 1:1000; Cell Signaling Technology, catalog number: 9936T), p-MAPK (ERK1/2) antibody (diluted at 1:1000, Cell Signaling Technology, catalog number: 5235LF), p-TLR4 antibody (diluted at 1:500; Abcam, catalog number: ab13556) and GADPH antibody (diluted at 1:1000 dilution; Abcam, catalog number: ab9485). Polyclonal goat anti-rabbit antibody (Cell Signaling Technology) was used as a secondary antibody (at room temperature for 2 h), which was imaged with ECL chemiluminescence reagent (Amersham) and detected by Western blot (Millipore).
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