Nusieve gtg low melt agarose

Manufactured by Lonza

NuSieve GTG low-melt agarose is a laboratory product designed for use in gel electrophoresis. It is a type of agarose, a polysaccharide derived from red algae, that has a lower melting point compared to standard agarose. This low-melt property allows for easier handling and manipulation of the gel during the electrophoresis process.

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Lab products found in correlation

Truseq small rna sample prep kit, by Illumina (1 mentions) Cellmask, by Thermo Fisher Scientific (1 mentions) Ixon emccd camera, by Oxford Instruments (1 mentions)

Spelling variants of this product

Agarose (75 citations) NuSieve GTG Agarose (33 citations) NuSieve GTG (11 citations) Low-melt agarose (7 citations) NuSieve (5 citations) NuSieve Agarose (3 citations)

2 protocols using nusieve gtg low melt agarose

Exosomal Small RNA Profiling from LCLs

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Small RNA from TRIzol-lysed LCLs and exosomes was isolated from 1 µg total RNA and analyzed for integrity using a Bioanalyzer 2100 Total RNA 6000 Nano Kit. cDNA libraries were produced using the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA) per manufacturer’s protocol, with the following modifications: (1) final PCR amplifications were 16 cycles for exosome cDNA and 14 cycles for cell cDNA, (2) instead of gel purification using PAGE, PCR-amplified small RNA libraries were gel-purified with 3% NuSieve GTG low-melt agarose (Lonza, Rockland, NY). Target bands of 147–157 nt, containing an adapter sequence of 125 nt, were excised and column purified (Qiagen, Germantown, MD). All cDNA libraries were indexed with Illumina adapters.

Tsang E.K., Abell N.S., Li X., Anaya V., Karczewski K.J., Knowles D.A., Sierra R.G., Smith K.S, & Montgomery S.B. (2016). Small RNA Sequencing in Cells and Exosomes Identifies eQTLs and 14q32 as a Region of Active Export. G3: Genes|Genomes|Genetics, 7(1), 31-39.

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Live Imaging of Basement Membrane Dynamics

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Live imaging was performed as described (Prasad et al., 2007 (link)), with the following modifications. Dissected egg chambers were placed on a pad of 0.4% NuSieve GTG low melt agarose (Lonza) in live imaging medium, and follicle cell membranes marked with CellMask (1:1000, Molecular Probes) or UAS-mCD8-RFP. The coverslip was cushioned with vacuum grease at each corner. For confocal imaging of BM fibril formation, a region of the Col IV-GFP in the BM was photobleached with a 488nm laser at 100% power for 10 iterations, 10 minutes prior to imaging. TIRF movies were taken on an Olympus IX-50 microscope equipped with an iXon EMCCD camera (Andor) and a 100× objective fitted with through-the-objective TIRF illumination. TIRF photobleaching was achieved by exposing the BM to the 488nm TIRF laser at 50% power for ~2 minutes, 10–15 minutes before imaging. Movies were processed using ImageJ.

Isabella A.J, & Horne-Badovinac S. (2016). Rab10-mediated secretion synergizes with tissue movement to build a polarized basement membrane architecture for organ morphogenesis. Developmental cell, 38(1), 47-60.

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