Dextran sulfate sodium (DSS, MW 36000-50000) was obtained from MP Biomedicals (CA, USA). The myeloperoxidase (MPO) assay kit was obtained from Jiancheng Bioengineering Institute (Nanjing, China). Zonula occludens protein 1 (ZO-1) and Occludin antibodies were obtained from Affinity (Liyang, China). FITC anti-CD4, PE anti-Foxp3, and PE anti-IL-17A antibodies were obtained from Biolegend (CA, USA). CD3e antibody, APC anti-CD25 antibody, Foxp3/transcription factor staining buffer set and IC fixation buffer were obtained from eBioscience™ (CA, USA). CD16/CD32 antibody was obtained from BD Biosciences (CA, USA). STAT3 and phospho-STAT3 (p-STAT3) antibodies were obtained from Cell Signaling Technology (MA, USA). The QuantiCyto® Mouse TNF-α enzyme-linked immunosorbent assay (ELISA) kit was obtained from NeoBioscience (Shenzhen, China).
Cd3e antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The CD3e antibody is a laboratory reagent used for the detection and identification of CD3e, a protein component of the T-cell receptor complex. This antibody can be used in various immunological techniques, such as flow cytometry, to study T-cell populations and their functions.
Automatically generated - may contain errors
Lab products found in correlation
Ic fixation buffer, by Thermo Fisher Scientific (1 mentions)
Anti il 17a pe, by BioLegend (1 mentions)
Anti foxp3 pe, by BioLegend (1 mentions)
Occludin, by Affinity Biosciences (1 mentions)
Phospho stat3 p stat3, by Cell Signaling Technology (1 mentions)
Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)
Cd16 cd32 antibody, by BD (1 mentions)
Myeloperoxidase mpo assay kit, by Nanjing Jiancheng (1 mentions)
Anti cd4 fitc, by BioLegend (1 mentions)
Anti cd4 apc, by Thermo Fisher Scientific (1 mentions)
Cd28 antibody, by Thermo Fisher Scientific (1 mentions)
7 aad, by Thermo Fisher Scientific (1 mentions)
Mouse cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions)
2 protocols using cd3e antibody
1
Dextran Sulfate Sodium Induced Colitis Assay
2
Suppressive Effects of iMDSCs on CD4+ T Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The CD3e antibody (5 μg/ml, eBioscience) was coated in 96-well plates at 100 μl per well. CD4+ T cells were isolated from normal mouse spleens using a mouse CD4+ T-cell isolation kit (Miltenyi, Germany). The sorted CD4+ T cells were stained with 1 μM 5, 6-carboxycein diacetate succinimidyl ester (CFSE, Invitrogen). Next, the CD28 antibody (5 μg/ml, eBioscience) was then added. Different ratios of CD4+ T cells (1 × 105/well) and sorted iMDSCs were co-cultured in 96-well plates for 72h. After co-culture, anti-CD4-APC and 7-AAD (eBioscience) were used to stain the cells, and the degree of CFSE decay in 7-AAD-CD4+ T cells was determined by flow cytometry. In the meantime, the proliferation index (PI) of CD4+ T cells was calculated as the ratio of the percentage of cells co-cultured with iMDSCs to cells co-cultured without iMDSCs, multiplied by 100 %.
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