Mouse ldh elisa kit

Five days after DOX injection, blood samples were collected from the retro-orbital plexus of mice. The mouse cTnI ELISA kit (#CSB-E08421m) and mouse LDH ELISA kit (#CSB-E17733m) were purchased from CUSABIO. Plasma cardiac troponin I (cTnI) and lactate dehydrogenase (LDH) were detected using commercial kits according to the manufacturer's instructions. Optical density values were obtained at 450 nm using a BioTek Synergy HT Multi-Mode microplate reader. To evaluate cardiomyocytes in vitro, creatine kinase (CK) was detected using a CK assay kit (A032-1-1, Nanjing Jiancheng Bioengineering Institute).

Heart tissues were homogenized in iced PBS, and homogenates were centrifuged at 4800 g for 20 minutes to isolate the supernatant. Myocardial lactate dehydrogenase (LDH) and creatine kinase- (CK-) MB were detected by using the commercial kits. The mouse LDH ELISA kit was provided by CUSABIO (#CSB-E17733m, Wuhan, China). The CK-MB assay kit (#A032-1-1) was obtained from the Nanjing Jiancheng Biological Engineering Research Institute.
The levels of glutathione (GSH), malondialdehyde (MDA), 3-nitrotyrosine (3-NT), protein carbonyl, total SOD activity, tumor necrosis factor-α (TNF-α) level, and interleukin- (IL-) 6 level were determined using commercial kits based on the manufacturer's instructions. GSH assay kit (#A061), MDA assay kit (A003-1-2), and the total SOD activity (A001-3-2) were provided by the Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). 3-NT competitive ELISA (#ab113848) was obtained from Abcam. The protein carbonyl ELISA kit was provided by Abnova (#KA6397, Taipei, Taiwan). The TNF-α Mouse ELISA kit (#BMS607-3TEN) was provided by Invitrogen, and the IL-6 (mouse) ELISA kit was provided by Biovision (#K4795). All assays were performed in triplicate.