Rhizopus oryzae

Commercially available sutures of P4HB (Monomax^® USP 1) were kindly supplied by B. Braun Surgical S.A.U. Weight and number average molecular weights of Monomax^® samples were 215,000 and 68,000 g/mol, as determined by GPC.
Pseudomona cepacia and Rhizopus oryzae enzymes with a specific activity of 40.0 and 55.7 U/mg solid, respectively, were obtained from Sigma-Aldrich (Madrid, Spain). All reagents, citric acid, phosphoric acid, chloride acid, boric acid, sodium hydroxide, sodium azide, and chloroform (CHCl₃) were supplied by Fisher Chemical (Hampton, NH, USA).

Hydrolysis reactions were performed by Aspergillus niger (product number 62301), Candida rugosa (product number 62316), Rhizopus oryzae (product number 62305), Rhizopus niveus (product number 62310) and Rhizomucor miehei (formerly known as Mucor miehei, product number 62298) free lipases obtained from Sigma–Aldrich (Munich, Germany). Seven oils, i.e., olive (refined; product number O1514), linseed (product number 430021), peanut (product number P2144), grape seed (product number W233218), rapeseed (product number 83450), almond (product number 63445) and menhaden fish (refined; product number F8020) oils, were involved to the assay. All oils were purchased from Sigma–Aldrich (Munich, Germany). PA (5.30–25.23%) was a major saturated fatty acid component in the oils used as determined by gas chromatography (GC) [46 (link)]. Concerning unsaturated fatty acids, OA (5.12–80.05%) and LA (1.91–23.79%) were among the major components of the oils. In addition, ALA (58.33%) in linseed oil and EPA (12.11%) and DHA (5.47%) in menhaden fish oil were also predominant fatty acid components. Individual fatty acids, i.e., EPA and ALA, were obtained from VWR (Debrecen, Hungary).