Caspase inhibitor z vad fmk

Manufactured by Selleck Chemicals

Caspase inhibitor Z-VAD-FMK is a broad-spectrum caspase inhibitor that irreversibly binds to the catalytic site of caspase enzymes. It is commonly used in various research applications to study apoptosis and cell death processes.

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Lab products found in correlation

Transdetect annexin 5 fitc pi cell apoptosis detection kit, by Transgene (1 mentions) Guava easycyte flowcytometry system, by Merck Group (1 mentions) Cell counting kit 8 assay kit, by Beyotime (1 mentions)

2 protocols using caspase inhibitor z vad fmk

Apoptosis Analysis of A431 Cells

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A431 cells were seeded on a 60-mm dish and transfected with siNC, siHOXA9_01, or siHOXA9_02 and cultured for 48 h. TransDetect Annexin V-FITC/PI cell apoptosis detection kit (TransGen Biotech) was applied according to instructions. Cell apoptosis was detected and quantified using a Guava easyCyte Flow Cytometry System (Merk Millipore) by the sum of both bottom and top right quadrants of the plots, e.g., including both early and late apoptotic cells. Caspase inhibitor Z-VAD-FMK (50 μM, Selleck) treatment was performed in cSCC cell lines (A431, HSC-1) for 48 h after transfection of HOXA9-expressing plasmid.

Zhou L., Wang Y., Zhou M., Zhang Y., Wang P., Li X., Yang J., Wang H, & Ding Z. (2018). HOXA9 inhibits HIF-1α-mediated glycolysis through interacting with CRIP2 to repress cutaneous squamous cell carcinoma development. Nature Communications, 9, 1480.

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Cell Viability Assay with Chemical Inhibitors

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Cell survival assay was performed using the Cell Counting Kit-8 Assay kit according to the manufacturer's instructions (C0037, Beyotime). ESCs underwent pre-treatment with 50 mm Caspase inhibitor Z-VAD-FMK (S7023, Selleck) and 30 mm RIP inhibitor Necrostatin-1 (S8037, Selleck) along with 0.5 mm 4OHT for 2 days, followed by re-plating for 4 days.

Wu K., Liu H., Wang Y., He J., Xu S., Chen Y., Kuang J., Liu J., Guo L., Li D., Shi R., Shen L., Wang Y., Zhang X., Wang J., Pei D, & Chen J. (2020). SETDB1-Mediated Cell Fate Transition between 2C-Like and Pluripotent States. Cell reports, 30(1).

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