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2 protocols using para red

1

Analytical Standards for Colored Compounds

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Acetonitrile (ACN), methanol, and formic acid (99.5%) (all HPLC grade) were provided by J.T. Baker (USA). Dimethyl sulfoxide (DMSO), hexane, acetone, ethyl acetate, ethanol, chloroform, and analytical standards of Sudan I, Sudan I-d5, Sudan II, Sudan III, Sudan IV, Sudan Red 7B, Sudan Red G, Sudan Orange G, Citrus Red, toluidine Red, and Para-Red were purchased from Sigma-Aldrich (Germany). Water was purified through a Milli-Q plus system from Millipore (USA) and PTFE syringe filters (0.2 µm, 25 mm) were received from Restek (USA).
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2

Quantitative Analysis of Synthetic Dyes

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New Coccine, Indigo Carmine, Erythrosine, Tartrazine, Sunset Yellow FCF, Fast Green FCF, Brilliant Blue FCF, Allura Red AC, Amaranth, Sudan II, Sudan IV, Dimethyl Yellow, Fast Garnet GBC, Para Red, Sudan Orange G, Sudan Red 7B, Sudan Red B and Sudan Red G were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sudan I was purchased from Tokyo Chemical Industry (Tokyo, Japan). Sudan III was purchased from Kanto Chemical (Tokyo, Japan). Methanol of HPLC grade was obtained from Mallinckrodt (Paris, KY, USA). Dimethyl sulfoxide (DMSO), acetonitrile, and acetic acid of HPLC grade were obtained from Merck (Darmstadt, Germany). Ammonia acetate was from Nacalai Tesque (Kyoto, Japan).
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