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Monoclonal anti flag m2 fitc

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Monoclonal anti-Flag M2-FITC is a laboratory reagent used for the detection and purification of proteins tagged with the FLAG epitope. It consists of a monoclonal antibody specific to the FLAG tag, conjugated to the fluorescent dye FITC (Fluorescein Isothiocyanate). This product can be used in various applications such as immunoprecipitation, Western blotting, and flow cytometry to identify and isolate FLAG-tagged proteins.

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Lab products found in correlation

Topro, by Thermo Fisher Scientific (2 mentions) Plan apochromat oil objective, by Zeiss (2 mentions) Mouse monoclonal antibodies against smith antigen snrnp family y12, by Abcam (2 mentions) Lsm 510 meta confocal laser scanning microscope, by Zeiss (2 mentions)

Close spelling variants of this product

Anti-FLAG M2-FITC Monoclonal FLAG M2 Anti-FLAG M2 Anti-FLAG monoclonal FLAG M2 Anti-FLAG FITC M2 monoclonal Anti-FLAG

2 protocols using monoclonal anti flag m2 fitc

1

Immunofluorescence Analysis of U2AF1 Mutant

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Constructs containing the S34F mutant allele were generated by site-directed mutagenesis of p3X-Flag-U2AF1. 293T cells were transfected with either p3X- Flag-U2AF1 (WT) or p3X-Flag-U2AF1 (S34F). 24 hours later, the cells were fixed in 2-4% formaldehyde-PBS for 20 min at room temperature, and then washed with PBS. Cells were permeabilized with 0.5% (wt/vol) Triton X-100/PBS for 10 minutes and blocked with 1% goat serum/0.3%TritonX-100/PBS for 1 hour. The following primary antibodies were used for fluorescence microscopy: mouse monoclonal antibodies against Smith Antigen snRNP family (Y12; Abcam) or U2AF2 (Sigma), and Alexa Fluor 555-conjugated anti-mouse (Sigma) as a secondary antibody. Monoclonal anti-Flag M2-FITC (Sigma) used to detect Flag-tagged U2AF1 and TOPRO (Life Technologies) used as a nuclear counterstain. Images were acquired using a Zeiss LSM510 Meta laser scanning confocal microscope (Carl Zeiss, Thornwood, NY) equipped with a 63X, 1.4 numerical aperture, Zeiss Plan Apochromat oil objective at 2.5 zoom and captured using Zeiss LSM510 software.
Okeyo-Owuor T., White B.S., Chatrikhi R., Mohan D.R., Kim S., Griffith M., Ding L., Ketkar-Kulkarni S., Hundal J., Laird K.M., Kielkopf C.L., Ley T.J., Walter M.J, & Graubert T.A. (2014). U2AF1 Mutations Alter Sequence Specificity of pre-mRNA Binding and Splicing. Leukemia, 29(4), 909-917.
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2

Immunofluorescence Analysis of U2AF1 Mutant

Check if the same lab product or an alternative is used in the 5 most similar protocols
Constructs containing the S34F mutant allele were generated by site-directed mutagenesis of p3X-Flag-U2AF1. 293T cells were transfected with either p3X- Flag-U2AF1 (WT) or p3X-Flag-U2AF1 (S34F). 24 hours later, the cells were fixed in 2-4% formaldehyde-PBS for 20 min at room temperature, and then washed with PBS. Cells were permeabilized with 0.5% (wt/vol) Triton X-100/PBS for 10 minutes and blocked with 1% goat serum/0.3%TritonX-100/PBS for 1 hour. The following primary antibodies were used for fluorescence microscopy: mouse monoclonal antibodies against Smith Antigen snRNP family (Y12; Abcam) or U2AF2 (Sigma), and Alexa Fluor 555-conjugated anti-mouse (Sigma) as a secondary antibody. Monoclonal anti-Flag M2-FITC (Sigma) used to detect Flag-tagged U2AF1 and TOPRO (Life Technologies) used as a nuclear counterstain. Images were acquired using a Zeiss LSM510 Meta laser scanning confocal microscope (Carl Zeiss, Thornwood, NY) equipped with a 63X, 1.4 numerical aperture, Zeiss Plan Apochromat oil objective at 2.5 zoom and captured using Zeiss LSM510 software.
Okeyo-Owuor T., White B.S., Chatrikhi R., Mohan D.R., Kim S., Griffith M., Ding L., Ketkar-Kulkarni S., Hundal J., Laird K.M., Kielkopf C.L., Ley T.J., Walter M.J, & Graubert T.A. (2014). U2AF1 Mutations Alter Sequence Specificity of pre-mRNA Binding and Splicing. Leukemia, 29(4), 909-917.
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